Redlight

N=20

Experiment group = A

bluelight

N=20

Experiment group = A

Gustatory preference under redlight

Gustatory preference under bluelight

It’s not yet confirmed, that the 3IY treatment is working

To see whether the initial preference but also the preference shift, observed for flies expressing the optogenetic Chrimson-Channel in neurons from the PPM2 cluster (tested for preference in JoyStick and T-Maze), does depend on dopamine signaling, I want to repeat these experiments with dopamine depleted flies. For this I plan to use 3-Iodo-Tyrosine, which should reduce dopamine production. To verify the effect 3IY I conducted two sets of experiments, testing locomotion and geotaxis. Successfully depleting dopamine should decrease locomotion and performance in geotaxis assays. At this point both experiments have the same outcome, that the tested method to apply 3IY does not work.

Locomotion in open field arena

Geotaxis in Benzer Counter-Current

Gustatory preference under redlight

Gustatory preference under redlight

I managed to write some code that shortens Tina’s optomotor periods and then adjusted the metadata between our experiments so that the script would run without any errors. So this is what our combined tests of expressing the foraging RNAi in all neurons looks like:

The full dataset is also available on the UR publication server.

CantonS under bluelight

Preference Index: (Pur-Salz)/(Pur+Salz+Neutral)

Stichprobengröße N: 20 jeweils

CantonS under redlight

Preference Index: (Pur-Salz)/(Pur+Salz+Neutral)

Stichprobengröße N: 20 jeweils