Joystick results of ATR and NOATR Control groups and ATR Sifa-G4 for red light.

Joystick results of ATR and NOATR Control groups and ATR 13.0273-G4 for yellow light.

These are results of ATR and NOATR Control groups and first experiments of SS5699 ATR with yellow light.

New results added for red light, with ATR, GR28bd-G4XTrpA1-G4, 13.0273-G4, Sifa-G4.

New experiments added for yellow light, ATR and NOATR Control group, ATR Sifa-G4 and ATR 13.0273-Gal4.

Control group with ATR, Red light, 7.7 Volt

Results of Milis JoyStick experiment.

At the beginning of last week, the flies in the optogenetics rescreen seemed to behave very different compared to all the weeks before: groups that kept the lights on now switched it off and vice versa. There was also some of that in the control flies, but to a lesser extent. This is what the last training PIs looked like for the seven groups before last week:

The data from last week then looked like this:

Especially the two groups that did receive ATR reversed their previous screen results. Potentially, reduced concentration of ATR may have reduced the effect of light which may have led to the reversal of the effects.

I’ve started rescreening Christian’s most consistent dopaminergic lines with the positive controls at the joystick machine, to see if everything works. These flies express the optogenetic channel Chrimson in Gr28bd and TrpA1 heat sensitive neurons, so they should use the joystick to switch the light off.

I tested three rounds of three flies each and I got the impression that it worked while I was looking at the flies. The data didn’t look that great, however:

Setting up the experiments, I noticed that the middle setup didn’t really focus the light on the head of the fly very well. This shows in the raw performance index data:

The two middle flies (row #2 and #8) have positive preferences, but never avoid the light, while nearly all other flies do avoid the light to various extents, even if they start out with a positive preference. So I deleted these two flies to see how the data would look then:

I decided that this should be good enough. I improved the middle setup to focus the light better on the fly’s head. Hopefully it will improve the PIs of the flies placed there now.