I managed to write some code that shortens Tina’s optomotor periods and then adjusted the metadata between our experiments so that the script would run without any errors. So this is what our combined tests of expressing the foraging RNAi in all neurons looks like:

The full dataset is also available on the UR publication server.

This is the final graph of the experiments knocking down PKG (foraging) in operant self-learning:

The data have been automatically uploaded via the new script and can be found on the University’s publication server.

This was supposed to be a reproduction of Tina’s experiment:

While in my dataset, the knock-down flies are still performing the worst of all three groups, it is not quite as clear-cut as in Tina’s results. Then again, her knock-down flies maybe were slightly too low. Now pooled with mine that were slightly to high, maybe this is what we should expect: a clear zero. We should pool the data and see how it looks.

Getting more and more flies, but only one group looks unambiguous. The other two not so much: