Recent wtb test

There often seems to be a strong preference for a side. The laser was off.

Flies often seem to have a strong preferenc for a side. In the imiage below the top fly was punished for left torque and survived, the fly below was punished for the right torque side and died.

Trails with drift

I’ve started again to test the set-up after repairs. It does not seem to be perfectly stable ,with some drift in the positive direction. Most often, it seems that the amount of drifting would be fine for measuring.

Therefor I tried measuring some flies. A problem now is a very negative baseline, when the fly stops flying. Also the response for left and right seems not to be equal.

So now I’m trying to get the line close to 0, so that I have conclusive results if the set-up if usable at the current state.

2 examples of drift:

The repetition of the knockout experiment in adult flies indicates that FoxP is not required for learning in adult flies. (Exp = elav tubGal80>Cas9gFoxP)

I wanted to expand and look into some general behavior of the mbon-2 flies. Mostly as a complement to the already existing data.

Knockout of FoxP in the PCB. Flies are still able to learn.

I experienced some issues with the first batch of the UAS-TNT control flies, theyh ad a very low learning curve. The second batched looked fine but the still have a slightly lower learning PI during Test1 than I want.

Mean or median?

A highly hypothetical scenario of how the distribution of dwelling times could be. Even if unrealistic, it still illustrates the problem of using median instead of mean.

Plotting the dwelling times as means

Grouped analysis of dwelling times

Cumming estimation plot