Knockout of FoxP in the PCB. Flies are still able to learn.

• Halfway in analyzing the Buridan data altogether (not in two batches)
• Maxiprepped plasmid for making FoxP protein at the klinikum
• Writing the thesis a little bit
• bought Fas-II antibody

________________________________________________________

• data 1): FoxP-iB Heterozygous/Homozygous comparison with Stinger-GFP. This time i was cautious with everything: fly all the same age and sex, same larvae density, same number of copies of GFP

I can not detect any difference between homozygous and heterozygous mutants. I also counted cells in IMARIS and I detected no difference in number or distribution.

• data 2): FoxP-iB Heterozygous/Homozygous comparison with CD8-GFP. This time i was cautious with everything: fly all the same age and sex, same larvae density, same number of copies of GFP

I can see some differences but i do not know how to quantify/explain them. Also I am not sure if it is a problem of dissection/mounting. I am not convinced.

• data 3): nc82 staining on homo/hetero FoxP-iB. I do not see differences

Problem: I haven’t managed yet to make the FasII antibody work:

I have used a 1:10 concentration of primary antibody for one night and a 1:100 concentration of secondary antibody for 4 hours. I will ask someone

In the picture I have increased the gain a lot just to be sure. I could not see anything

What magnitude of drift is still ok?

1. Crossed the flies for the course
2. Almost finished the Buridan for C380
3. Worked on the manuscript and supplemetary figures
4. Done the FoxP reaction on induced embryo again because I was afraid after the different results Andi had last time