T4/T5 lines test crossings
Different T4/T5 driver lines are crossed with different effector lines to assess the viability of the F1 progeny
T4/T5 lines test crossings
Different T4/T5 driver lines are crossed with different effector lines to assess the viability of the F1 progeny
TH Driver lines larval expression verification
TH-D’-Gal4>UAS-mCD8::GFP; aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil
NP1568-Gal4>UAS-mCD8::GFP (adult brains); aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil
TH Driver lines larval expression verification
NP1568-Gal4/CyO>UAS-mCD8::GFP; aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil
TH Driver lines larval expression verification
NP0047-Gal4/CyO>UAS-mCD8::GFP; aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil
TH-D’-Gal4>UAS>>mCD8::GFP,UAS>>mCD8::GFP; aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil
TH-D1-Gal4>UAS>>mCD8::GFP,UAS>>mCD8::GFP; aGFP (rabbit), NC82 (aBrp, mouse), GAR AF 488, GAM AF 555; Leica SP8, 20x immersion oil
Optogenetic activation of Mushroom body Kenyon cells affects naïve salt avoidance in Drosophila larvae
The mushroom bodies (MBs) are known to be the most prominent olfactory memory center in Drosophila. The MB Kenyon cells receive olfactory (via projection neurons, PNs) and gustatory (via dopaminergic neurons, DANs) input. The coincident detection of these stimuli elicits then the formation of associative memory. However, little is known whether and how this high order brain center affects naïve behaviors such as olfaction and gustation. We use fruit fly larvae to test the innate salt avoidance in a simple choice assay. Drosophila larvae are tested for 3 minutes for their preference for 1.5M NaCl. We show that salt avoidance is enhanced upon optogenetic activation of the Kenyon cells. This effect was also observed after ablation of pPAM DANs with simultaneous optogenetic activation of the KCs. We were able to phenocopy this effect by optogenetic activation of TH-Gal4 positive dopaminergic neurons, however not after artificial activation of single DANs of the DL1 cluster. In contrast, larvae showed no salt avoidance after optogenetic activation of sNPF-Gal4 positive neurons. Neuropeptidergic and dopaminergic signaling are tightly involved in modulation of the MBs. However, it is not clear whether our observations depend on coherent neuronal pathways. Therefore, we use simultaneous manipulations of different components of the MB, dopaminergic and sNPF circuitries to unravel the neural mechanisms of modulation of naïve gustatory behaviors in the Drosophila larvae.
Salt avoidance
5 days old larvae (3rd instar) were tested for their salt (1.5 M) avoidance behavior. Dopaminergic neurons from the TH-D’ cluster were activated upon ChR2-XXL expression and blue light exposure, respectively.
Self-learning, 8 minutes training, elav-Gal4>UAS-for-RNAi
It seems that panneuronal downregulation of the expression of the foraging gene impairs yow torque learning when flies were trained for 8 minutes.
