Test of set-up after repair.
The signal is drifting again. Also adding a spacer between clamp and device is not solving it, but making the signal more noisy.
New problems with the set-up
The measuring device needed repairing, after aperently some glued part got loosloosened and produced no signal anymore. The divice is now producing a signal again, but it is shifted.
The direction of the fly seems to be the main thing. Same fly faceing to the laser (left) and away (right)
Search for neurons matching the FoxP expression pattern
I started looking at the FoxP expression pattern from Ottavia to see if i can identify the neurons.
Control cross
Control cross of UAS-Cas9;UAS-gFoxP with ELAV-GAL4;UAS-Cas9 is working. Flys are not able to fly and are walking poorly.
Control cross of Hs-Gal4 with UAS-Stinger is still not working.
UASCas9;UASgFoxP X ElavG4;TubGal80
Update on coditional FoxP manipulation. Either no manipulation at 18°c or switch to 30°c for adult flys.
Bottom: no switch in temperatur