on Monday, July 30th, 2018 1:52 | by Anokhi Kashiparekh
on Monday, July 23rd, 2018 1:55 | by Anokhi Kashiparekh
This week we worked on perfecting the fly hooking. Some of the data we got is as follows (Data from just one fly).
on Tuesday, May 15th, 2018 12:26 | by Christian Rohrsen
Confocal image MAX stack of one of the brains at 20x
and at 40x
In this link we have a video of a 3D stainning pattern zoomed_CC
on Wednesday, April 4th, 2018 3:38 | by Christian Rohrsen
After performing EMD to 6 fly traces of 20000 data points (that is 1000 sec flight) for each group (tntXwtb; c105;c232>tnt; c105;c232Xwtb). This data size was chosen to reduce computing time of the SMAP procedure. The EMD decomposes the trace into different time scales in nonstationary data. It seems that the nonlinear behavior occurs at the first IMF (the fastest time scale) and a bit in the second IMF. The potential conclusion to this is that the behavior of the the fly is only unpredictable at the fast movements whereas slow movements are very predictable. Nevertheless, to be cautious it could be that this fastest timescale is just noise, and that this noise is nonlinear. I would say that there is no difference at any time scale between groups (pay attention to the different ranges in the Y-axes), so the ring neurons R1, R3, R4d do not have any effect.
As a groundtruth I have used the same analysis pipeline for the traces in the uniform arena from Maye et al. 2007. Here the effect is even more pronounced at the fastest time scales. So I will conclude that this is real fly behavior and not noise that is shared among both setups: the Ping pong ball machine and the torquemeter.In order to gain more insights into the underlying flight structure I took one random flight trace to explain a few observations. The x-axis is the theta (that actually goes from 0-4 in steps of 0.2 and therefore we see the 21 points), in the y-axis is the correlation of the prediction to groundtruth. We see that IMF has a bigger slope, but not only that, also that its prediction correlation is around 0.88, whereas lower timescales prediction is basically perfect. That is, fast time scales are not only more nonlinear but also less unpredictable. This pattern is repeated in every fly measured
on Tuesday, March 27th, 2018 5:10 | by Christian Rohrsen
This is for the sake of playing and curiosity. I made out of these two traces a modelling of their flying trace in a 2D world. Direction is right wing amplitude – left wing amplitude and distance flown is dependent on the sum of both (more amplitude of both, more forward thrust). Funny enough, the second one looks kind of fractal, which is characteristic of chaotic behaviors. If there is any comment to add to this new visualization, all ears!
on Monday, January 29th, 2018 12:40 | by Christian Rohrsen
Some traces from this week just so that you have an idea how do they look like. To me they are not the optimal traces I expected. But one can see some signal there. I will start the screen hoping to get enough good traces without too much work.
what do you think is the best quality control for accepting a trace for the analysis or not. I was thinking the 3D mapping gives a good hint but without quantification.
on Monday, December 4th, 2017 1:02 | by Christian Rohrsen
This is another way of showing how the slope of the SMAP analysis varies with length. I have choped the time series in 4 chunks and saw what was the slope for the chunks and for the whole time series. I think this is the best statistical way of doing it, it should not depend on what line was tested. I cannot see any effect.
Here below is just to show what I found out in the code. What I thought that theta was controlling for nonlinearity in the model, for me it seems rather a control for under- overfitting in the model. So I might need to read the paper and see what do they say, and if it is the same as what I found out in the code.
on Monday, November 27th, 2017 2:43 | by Christian Rohrsen
So this is the final graph assuming that 20Hz is the sampling rate. Sathish was not sure what it was and he said he will check.
To have a better overview how the length of the flight compares with the slope obtained from the SMAP. There is no big correlation whatsoever with around 100 flies. Sathish found this in his thesis with data from seventy something WTB, but to me it seems like an anecdotal result.
Here the same as above, just for showing the fit line.
on Monday, November 20th, 2017 1:47 | by Christian Rohrsen
This are the results of all of the flies analyzed from Sathish. I just need to know the frequency of the acquisition to exclude the ones that are below 6 minutes.
on Monday, November 6th, 2017 1:37 | by Christian Rohrsen
I have analyzed the c105+c232 > tnt data from Sathish. There are a total of 43 flies, although many of them only flew for a few minutes, and therefore should be discarded. Below all of the individual fly scores. I need to analyse now the other groups. This is btw the modified data set, whatever that means for Sathish.
This is a video of the projection from the torque data from Maye et al. 2007. The spikes are not that well sorted in this case as in the Strokelitude. I guess this is because the spikes do not look so smooth.