Four minutes not enough
on Friday, September 29th, 2023 4:20 | by Björn Brembs
Eight minutes of yaw torque training work just fine for both wild type and mutant flies:

Reducing the training to four minutes is not enough for wild type flies:

Now it will be exciting to see if the mutants still do what they did many years ago: learn better than wild type.
Category: Operant learning, operant self-learning | No Comments
Little by little
on Monday, July 24th, 2023 1:59 | by Björn Brembs
Despite very warm weather, some flies did fly, even though the learning performance of the control flies was really poor. At least for now, it looks like all stocks are learning and that rut and rsh flies learn at least equally well as the Berlin flies. I’ve also managed to fix the positive preference problem:

Category: operant self-learning, Radish | No Comments
Creating gRNAs via PCR
on Friday, July 7th, 2023 6:38 | by Isabel Stark


The template pCFD6 was used with a concentration of 640 pg/µl.


The template pCFD6 was used with a concentration of 64 pg/µl (1:10 dilution).


The template pCFD6 was used with a concentration of 64 pg/µl (1:10 dilution).
50µl of 5xQ5 High GC Enhancer was added to the PCR mix.

The template pCFD6 was used with a concentration of 128 pg/µl (1:5 dilution).
50µl of 5xQ5 High GC Enhancer was added to the PCR mix.


The template pCFD6 was used with a concentration of 640 pg/µl.
The Phusion DNA Polymerase was used instead of the Q5 High-Fidelity DNA Polymerase.
Category: genetics, Memory, Operant learning, operant self-learning, Radish, Uncategorized | No Comments
It’s a start
on Friday, June 16th, 2023 4:42 | by Björn Brembs
The first few rutabaga and radish mutants have been measured and so far it looks good: standard duration yaw torque learning – the situation all three groups should learn.

Category: Operant learning, operant self-learning | No Comments