T-Maze experiments : screen results as on 25-06-2018

Yellow 1 (Positive Control):  Gr28bd-G4, TrpA1-G4

Parameters:
Light: intensity (500 Lux side, 1000 Lux bottom)

frequency = 20Hz

Delay = 1 ms

Duration = 9.9 ms

volts = 6.4

Initial screen results

Yellow 1 (Positive Control):  Gr28bd-G4, TrpA1-G4

Light: intensity (500 Lux side, 1000 Lux bottom), frequency (20Hz)

New experiments

I was trying to increase the differences between flies with clipped and intact wings, putting a paper covering the LEDs.

Results are not as good as I expected. The differences are lower than experiments done without this paper between LEDs and flies. I did experiments only with the big white LEDs (the ones which showed better results).

Comparison between voltages and size of white LEDs

I continued doing experiments with big and small white LEDs, and now I think I have done enough experiments to compare them.

These are the final results, which seem to indicate that big white LEDs using 2,6V are the best ones to continue with the experiments.

New phototaxis results

I am trying to find which are the best LEDs and voltages to do the phototaxis experiments.

For that, I was doing experiments with big and small white LEDs (the big ones are much more brighter than the other ones), and with two different voltages: 2,6V and 3V.

This are the results that I have obtained:

As we can see in the pictures, the best results are obtained when we use a voltage of 2,6, and for now big LEDs seem to be better than the smaller ones (we find more differences between flies with clipped wings and flies with intact wings).

But the number of experiments I have done are still not enough to make a good comparison between LEDs size and voltage.