JoyStick results for 13.0273 and SIFa

on Monday, January 20th, 2025 11:05 | by

13.0273 (Red)

For all Figures the left side displays all 10 testing and training periods for the JoyStick experiment. The right side compares the PIs of the last training period between the groups. Graphics indicate whether red or yellow light was used.

13.0273 (Yellow)

SIFa (Red)

SIFa (Yellow)

T-Maze CIs and JoyStick Last Training PIs for yellow and red light.

on Tuesday, January 7th, 2025 1:45 | by

StrainDA neuronsReference
SS56699PPL1-FBHulse et al. eLife 2021
TH-D-DBD; TH-C-ADPPM2Xie et al. Cell Reports 2018
TH-FLP-p10; 64H06PPM3Xie et al. Cell Reports 2018

The left-hand side of the figure displays the choice indices (CIs) for the different groups tested for 1 minute in the T-Maze setting. On the right-hand side, the preference indices (PIs) for the final training period in the JoyStick setting are shown. Since flies of the TH_Flp_p10;64H06 line were not blind, they could not be tested in the T-Maze setting. The upper part of the figure refers to experiments conducted with yellow light, while the bottom part to experiments with red light, as indicated by the graphics.

In previous posts I referred to the different dopaminergic neurons (DA neurons) with the names of the driver lines used for the crossings. The table below the figure connects the fly strains to the targeted neurons and gives the reference. Gr28bd+TrpA1 target heat sensing neurons and acted as a control, since flies expressing the chrisom channel in these heat sensing neurons would avoid light activation. Flies were fed with all-trans retinal (ATR) for 2 days before the experiments, to enable light activation of the targeted neurons. For the negative control ethanol was used.

In the T-Maze experiments, flies were tested for 1 minute without prior exposure to light, whereas the JoyStick results reflect preferences after nine 1-minute training periods. Therefore, the T-Maze experiments should be repeated using longer testing periods. Additionally, PIs from the initial training periods in the JoyStick experiments will be included to allow for better comparison.

Hulse et al. eLife 2021: https://doi.org/10.7554/eLife.66039

Xie et al. Cell Reports 2018: https://doi.org/10.1016/j.celrep.2018.03.068

Joystick results Yellow light, testing with and Without food

on Monday, November 25th, 2024 1:31 | by

The positive control was tested once without food and once with food to observe its effect on general behavior, particularly on learning behavior. NF = No Food / WF = With Food

All Joystick Results Yellow

on Monday, November 11th, 2024 11:16 | by

All Joystick Results Red

on Monday, November 11th, 2024 11:14 | by

Joystick Results Yellow Light 29.10.24

on Tuesday, October 29th, 2024 2:19 | by

Joystick Results Red Light 29.10.24

on Tuesday, October 29th, 2024 2:14 | by

Final ‘Joystick’ test results

on Monday, January 29th, 2024 11:30 | by

Over the course, control and test lines were experimented with under ‘Joystick’. The test lines include 13:0273-Gal 4 line, SS56699 line, and TH-C-AD; TH-D-DBD line. The tests were done under red and yellow light conditions.

Red light conditions:

Yellow light conditions:

Verification TH-C-AD;TH-D-DBD

on Monday, September 11th, 2023 9:52 | by

Confocal images with anti-GFP and anti-Brp staining of TH-C-AD;TH-D-DBD > mCD::GFP fly brains: A: 4 PPM 2 DANs per hemisphere marked with blue circles, three clusters of Kenyon cell bodies per hemisphere marked with white arrows. B: Five visible cell bodies of unidentified neurons marked with a blue circle in the left hemisphere. The neurons project into the lobula plate and the medulla. Strong fluorescence of cell bodies of the Kenyon cells projecting into the Mushroom bodies is visible. C: Unidentified neurons projecting into structures outside of the optic lobes especially the ventral lateral protocerebrum.

Joystick Results for 2-minute-testing

on Tuesday, May 30th, 2023 1:31 | by

The following figures show the results of the joystick test using the same test line as before. In addition, the flies were fed not only ATR but also 3IY (3-iodo-L-tyrosine), an inhibitor of dopamine synthesis. This was done to determine whether the previously observed effect was due to dopamine alone and could therefore be suppressed by the inhibitor, or whether other factors also influenced the flies’ behaviour in the joystick test.