N= 30, p-value = 0.66

Rescue from forS in visual memory defects:

adult-limit for expression in fan-shape body (c205-Gal80ts)

increase for expression in ellipsoid body (c819-Gal80ts)

Rover,N= 36

Sitter, N= 28

WTB, N= 13

PKG affects K channels

Two ways to inhibitatePKG:

1

UAS-Gal4, RNAi

UAS-GCaMP3/OK6-GAL4; UAS-for RNAi/+

UAS-GCaMP3/OK6-GAL4; UAS-for RNAi/ UAS-for RNAi

2

PKG inhibitors:

1. KT 5823(10mM in DMSO)
2. (irrversible)

Rover, N=3

sitter, N=2

wtb, N=4

longterm, N=13 (old WTB excluded)

R,S self learning traces, and longterm memory trace

WTB self learning traces, longterm memory trace

R=30

S=20

longterm memory N= 22

control N= 6

longterm test:

1 from sitter,  8 from wtb, 3 from rover (total N = 12)

mean(test): 0.248          SD(test): 0.7

Longterm test Control

1 from sitter, 2 from rover (N=3)

mean(test):-0.386           SD(test):0.79

Two Sample:

Based on 50%(20%) of statistical power, the minimum sample size is 7 (17)for both group.

One Sample (assume control group has the average of PI is 0):

Based on 50% (20%) of statistical power, the minimum sample size is 22 (49)for both group.

https://www.dssresearch.com/KnowledgeCenter/toolkitcalculators/samplesizecalculators.aspx

Self learning test:

sitter N=5

wtb N= 4

There is the result of 20 measurements on PKG rover.

pretest:-0.45

train1:0.56

train2:0.71

test1:0.47

train3:0.83

train4:0.87

test2:0.39

After camera and punish laser adjusted, flies showed a high avoidence in the trail. Especially, when the fly was hit by punish laser in the first time of each training block. Unfortunately, there was a huge drift in the last trace after adjustment, but it shows a good avoidence in each training block.

Generally, PKG drosophila were flying much better when they hatched from normal temperature. There is the result from 10 PKG drosophila.

There is a short update on summerized previous PI. Still, PKG transgenic drosophila is hardly testable, and the results seems not so promising. I’m going to focus on testing WTB this week, to compare the difference of PI between WTB and PKG

There are two strains of drosophila prepared: PKG-rover(R), PKG-sitter(S). Each strain is supposed to test PI in 3 phases: no training, training, long term+no training. The following data show PKG-sitter flying trace and PI. Since there weren’t many flies in the first week, and they pause flying very often in the experiment, so there is only one single fly was tested successfully. Since the torque meter drifted on the next day, the long term result is quite debatable.