Complete Buridan

on Tuesday, March 3rd, 2020 5:44 | by

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Category: buridan, Foxp | No Comments

UASCas9;UASgFoxP X ElavG4;TubGal80

on Monday, March 2nd, 2020 1:33 | by

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Comparison FoxP and FoxP/+

on Monday, March 2nd, 2020 1:24 | by

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Supplementary figure S1 and S2

on Thursday, February 27th, 2020 11:13 | by

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Waiting for crosses to hatch

on Wednesday, February 26th, 2020 1:46 | by

  1. ato-Gal4 x conditional KO —> Removing FoxP in Dorsal Cluster Neurons for Buridan
  2. cmpy-Gal4 x conditional KO —> Removing FoxP in Protocerebral Bridge for Buridan
  3. temperature switch at embryo stage but shorter time —> for replicating the KO phenotype
  4. LexA + Gal4 together to analyze larval brain (I have always considered larvae except for this experiment, so I am doing it to be more complete)

Furthermore, for not loosing time this week I did:

  1. Leipzig presentation
  2. Wrote a bit of results for paper/thesis
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UASCas9;UASgFoxP X ElavG4;TubGal80

on Wednesday, February 19th, 2020 1:13 | by

Update on coditional FoxP manipulation. Either no manipulation at 18°c or switch to 30°c for adult flys.

Top: switch to 30°c for adult flys.
Bottom: no switch in temperatur
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Graphs new look

on Wednesday, February 19th, 2020 11:17 | by

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LexA construct qPCR

on Wednesday, February 12th, 2020 3:35 | by

First graph: FoxP-iB-Gal4
Second graph: FoxP-LexA
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Additional ELAV-Gal4>UAS-gRNA control

on Thursday, February 6th, 2020 5:23 | by

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Category: buridan, Foxp | No Comments

UASCas9;UASgFoxP X ElavG4;UASCas9 or ElavG4;TubGal80

on Monday, February 3rd, 2020 1:22 | by

First data of conditional FoxP temperature switch in adult flys

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