Weitian Sun

Laser power/angle adjustment

After camera and punish laser adjusted, flies showed a high avoidence in the trail. Especially, when the fly was hit by punish laser in the first time of each training block. Unfortunately, there was a huge drift in the last trace after adjustment, but it shows a good avoidence in each training block.

PKG rover from last week

Generally, PKG drosophila were flying much better when they hatched from normal temperature. There is the result from 10 PKG drosophila.

Update on PKG sitter/rover PI

There is a short update on summerized previous PI. Still, PKG transgenic drosophila is hardly testable, and the results seems not so promising. I’m going to focus on testing WTB this week, to compare the difference of PI between WTB and PKG

First glance at PKG effect on self-learning and long term memory

There are two strains of drosophila prepared: PKG-rover(R), PKG-sitter(S). Each strain is supposed to test PI in 3 phases: no training, training, long term+no training. The following data show PKG-sitter flying trace and PI. Since there weren’t many flies in the first week, and they pause flying very often in the experiment, so there is only one single fly was tested successfully. Since the torque meter drifted on the next day, the long term result is quite debatable.

Wild Type Drosophila memory ability test

There were 6 more flies were tested, and PI from two tests are focused this time.  The inconstancy in PI (#23, #33)  from test1 and test2 indicates the learning or memory function in wild type during test.

• PI from all 6 groups

• fly trace(#33)

• test1 (#33)

• test2(#33)

Wild type Drosophila flying trace

Wild Type Drosophila were used for single fly trace testing. The basic ideal is to record the spontaneous behavior (flying trace) after punishment training by heat. The followings are 3 subjects were test with performance index (PI).

•  wtb_08 (right punishment).

• wtb_10 (left punishment).

• wtb_14 (left punishment).

• Average.

PS: From Julien’s paper, he demonstrates that there are 7 blocks were included in PI ( one pretest:PI1; four training test: PI2, PI3, PI5,PI6; two memory test: PI4,PI7)