Verification TH-C-AD;TH-D-DBD
Line verification SS56699
After dissecting brains from the GAL4 driver line SS56699 with GFP staining and finding no fluorescence, I dissected them again and the immunohistochemical staining showed fluorescence. To check that the correct neurons were stained, I compared the images with the image in the paper by Hulse et al (2021; https://doi.org/10.7554/eLife.66039) . The three PPL1 dopaminergic dorsal fan-shaped body tangential neurons per hemisphere are stained, but there are some additional unidentified neurons (presumably PPM1 neruons) visible.
Hulse, B. K., Haberkern, H., Franconville, R., Turner-Evans, D., Takemura, S.-y., Wolff, T., Noorman, M., Dreher, M., Dan, C., Parekh, R., Hermundstad, A. M., Rubin, G. M., & Jayaraman, V. (2021). A connectome of the Drosophila central complex reveals network motifs suitable for flexible navigation and context-dependent action selection. eLife, 10, e66039. https://doi.org/10.7554/eLife.66039
Joystick Results for 2-minute-testing
The following figures show the results of the joystick test using the same test line as before. In addition, the flies were fed not only ATR but also 3IY (3-iodo-L-tyrosine), an inhibitor of dopamine synthesis. This was done to determine whether the previously observed effect was due to dopamine alone and could therefore be suppressed by the inhibitor, or whether other factors also influenced the flies’ behaviour in the joystick test.
Red light Joystick Results
The light used is red and has an intensity of 400 to 470 lux, the LEDs have a voltage of 7.5 volts. The control flies observed seem to show a stronger effect than in previous tests, which could be due to the use of a thinner fishing line (0.6 mm), which is easier to place correctly in the clamp of the joystick machine.
For the negative and positive control we used the line norpAP24;Gr28bd+TrpA1>Chrimson.