15 Oct Sathish’ log
on Monday, October 15th, 2012 6:43 | by Sathish K Raja
Well,
Since its lab notebook, I write in such a way that I could easily understand the tasks and rest could follow up. I will post figures with explanation and results whenever available.
Matlab:
1. working on the scripts to differentiate initial 2 min and last 2 min of self learning exp. to see the learning effect.
dataset suffix numbered in random fashion, unable to create handle to embed in struct field. Cell array conversion was possible but cant identify the fly specificities. Waiting to get updated version of data from Julien.
21 Hz corrected to 20 hz using interp1 method written and saved in home/character/Julien/
rest of code compiled and saved in same loc.
2. Buridan data – after analysis .csv file data is arranged on basis of activity and non activity.
wrote codes to extract each fly data out of .csv and place in .mat file to enable easy loop over. error due to presence of 2 activity log subsequently due to bursts inside activity matrix. troubled files isolated.
interval between each activity is considered as IAI . Even number logic is used in script. data(m,n)*ones(M)
sprintf(‘sol%d’,’iteration’) leaves only string ,convert using str2num and call the metafile.Yet to compile.
3.Altered the Grip code such that grip does not try to convert the location of activity(spikes) to time domains.saved as griburidan.m only used with buridan and pysolo data.yet to compile to function
Flies:
1.crosses under way for self learning with crowd control
2.c819 Gal4 arrived from Bloomington n quarantined.
Log end
Category: buridan, Spontaneous Behavior | No Comments
News from Neurofly
on Tuesday, September 11th, 2012 6:50 | by Julien Colomb
A lot of things I learned at the neurofly. First my old friend Benjamin is doing a great postdoc in Bruno van Swinderen lab. It was great to see his data on isoflurane effects and the correlation between sleep disorder and sensitivity to anesthesia.
More related to our interests, I learned that Paul Tchénio has now a prototype to visualize neuronal activity in about half of the fly brain at a decent frequency. It may be interesting to get in touch with him again.
Andre Fiala is doing/has already done the TDC-Flp construct and is using his own UAS-stop-TRPA1 line to get a subset of octopaminergic neurons. Christine will contact him soon to have more information and maybe start a collaboration with him. The student was not at his/her poster when we went there, and we could not talk directly to him/her.
I nice talk by F. Mohammad (Singapore) showed that centrophobism can be associated with anxiety: the majority of treatments used with mices (for instance immobilisation in a pipet tip) also induce more (or less for some treatments) centrophobisms in flies. I told him about the CeTrAn, he said he will look at it. (his flies were in a squared box, with their wings untouched).
Jhl21 (receptor for JH) change of larval behavior at the wandering stage (go slower and turn more): it acts at the NMJ to change the clustering of glutamate receptors. Similar thing may happen during the first hour of life of the fly, when they become phototactic ?
A nice poster from the Heisenberg’s lab show that flies do have a preferred 0 torque. Even when giving some closed loop drift, the histogram of torque suggest that the preference for the 0 torque is still there (non-uniform distribution around the +1 torque which stabilize the drift. It seems the distribution around the 0 torque is seen only if you have long enough data, I told them I would ask Satish to look at his distributions. This emphasizes why we need to be very careful in preparing the fly for our experiments…
I may have to look at the OK6 Gal4 line for motorneurons, and new RNAi lines seem to be available for PKC53e and FoxP…
I also talked to Flybase people, David was there. It seems the Buridan results should lead to 2 different entries: one linking each genotype to a dichotomic description of the phenotype (“mutant1” – “has larger”- “median_speed”) and one to the raw data and analysis. This will not be easy to automatize, but looks interesting.
I am probably forgetting a couple of things in addition to my discussion with Anette Schenk and her student Anna about FoxP..
LED arena set up guide updated under lab resources
on Friday, January 27th, 2012 12:24 | by Sathish K Raja
Simpler version of set up guide
Category: Spontaneous Behavior | No Comments