Simon Benmaamar

Larval food manipulation first evaluation

on Monday, February 1st, 2021 12:40

Food Patch Experiment

The development of larvae that grew up on food without yeast was delayed by about 2 days. Larvae grown on starch feed developed faster by a day.
The standard food was probably too dry.

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Power analysis and food manipulation recipes

on Monday, January 25th, 2021 11:50

Power analysis sample size

Food manipulation recipes

Food recipes

Fly food protocol: 1 l of water was brought to a boil in a cooking pot using an induction cooker. Then 17 g of agar-agar dissolved in 350 ml of water was added and also brought to the boil. Diastatic malt extract and sugar beet sirup were added to the agar-water mixture. After that, polenta, brewer’s yeast and soy meal dissolved in 350 ml water were added to the pot. The mush was boiled for about 10 minutes while stirring continuously. Finally some water was added and after a short cooling down 6 g Nipagin dissolved in water was added. The mash was then filled into vials.

Nutrient distribution

a) Quantity distribution

b) Percentage distribution

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Rover/Sitter Density Experiment

on Monday, January 18th, 2021 1:30

1. Food Patch Experiment

Control

There was a significant difference between the groups (t-test, p =0.01274)

Crowded

There was no significant difference between the groups (t-test, p = 0.2284)

2. Tracking

60 seconds

Control

There was a significant difference between the groups (t-test, p =0.000162)

Control

Crowded

There was no significant difference between the groups (t-test, p = 0.07565)

Crowded

45 seconds

Control

There was a significant difference between the groups (t-test, p =0.001252)

Control

Crowded

There was no significant difference between the groups (t-test, p = 0.2159)

Crowded

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Planned experiments

on Monday, December 21st, 2020 11:37

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Latest food patch experiments on Rover/Sitter larvae

on Sunday, December 20th, 2020 5:31

Food Patch Experiment

For Control larvae from the 11.12.2020 and 14.12.2020. There is nearly no difference between both groups.

Time of first movement function

Additional function to plot the time when the larva starts moving. The Data from last week was used

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Fly food analysis

on Monday, December 14th, 2020 11:54

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Further testing of the For Control Group

on

Food patch experiment

Barplot of the For Control Group with additional n=4 .

Distance tracking

The larvae were filmed for one minute. Larvae that reached the edge of the petri dish before one minute were discarded.
Video recording was started as soon as the larvae where placed on the agar plate.

Same data cut after 30 s.

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New experiments on Rover/Sitter larvae

on Monday, December 7th, 2020 12:29

Testing new tracking software: Kinovea

Barplot of the distance of the Canton-S WT larvae of last week. There is no big difference compared to the tracking with Biotrackr.

Food patch experiments on old Rover/Sitter larvae

ForS and ForR larvae raised on new food. There is a bigger difference between the groups.

Distance tracking of old Rover/Sitter larvae

The larvae were filmed for one minute. Larvae that reached the edge of the petri dish before one minute were discarded.
Video recording was started as soon as the larvae started moving.

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Distance tracking of larvae

on Monday, November 30th, 2020 11:57

Testing of the tracking and R Code

1) Small paper ball to simulate a larva, attached to a black string, and moved 2 cm by hand. 2) Measuring the pixel distance of a known real life distance (1 cm). 3) Plot of x and y coordinates shows trajectory of the object. 4) Bar plot of the real life distance shows that tracking and the R code worked .

Distance tracking of Canton-S wild type larvae

1) Larva on agar plate dyed with activated charcoal 2) Barplot of the distance of the Canton-S WT larvae

Problem finding the video time

Output of the csv file of the BioTracker.

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Further experiments on larval locomotion using Canton-S wild type flies

on Sunday, November 22nd, 2020 10:33

All experiments were done using 1 mm graph paper and a microscope to count the boxes more precisely.

Tracking of the larvae using Ctrax

Agar plate dyed with activated charcoal (2 g charcoal/ 1 l H2O)

Tracking with Ctrax software using the Burridan camera setup.

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