Phototactic flexibility – Neural substrates
In order to find which dopaminergic and octopaminergic neurons are related to light preference and the switch on it seen after clipping the wings, I decided to use a tool called CaLexA.
Here we can see my first attempt to use it. We can see the CNS from TH>CaLexA flies with and without wings.
I did not see any special signal in the brains, but I still have to play around a little bit more.
With Wings
Without Wings
Update: Looking for the DA & OA neurons involved in phototactic flexibility
These are the newst results of my screen. There are some interesting candidates, but it’s still too soon to conclued something.
Update of the DA screening. New experiments are in red (25°C) and blue (32°C).
Experiment finished.
Looking for the DA & OA neurons involved in phototactic flexibility
After using TH-G4 and TDC2-G4 to prove that dopamine and octopamine are involved in phototactic switch after wing clipping, I decided to find which neurons in particular are playing this particular role. Therefore, I started a few small screens with the GAL4s and LexAs available for these groups of neurons. I crossed the drivers to shibire to assess their role. For every graph blue means RT, and red means 32°C.
TH subsets-GAL4
Dopaminergic neurons -GAL4
CS substrains in phototaxis
I started testing the different CS substrains that we have here (CS from the paper https://f1000research.com/articles/3-176/v1 + CS Regensburg). I’ve decided to start with Benzer Paradigm. This is what I have so far.
Updated:
