E. Axel Gorostiza

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Phototactic flexibility – Neural substrates

on Monday, November 23rd, 2015 2:29

In order to find which dopaminergic and octopaminergic neurons are related to light preference and the switch on it seen after clipping the wings, I decided to use a tool called CaLexA.

Here we can see my first attempt to use it. We can see the CNS from TH>CaLexA flies with and without wings.

I did not see any special signal in the brains, but I still have to play around a little bit more.

With Wings

TH CaLexA With Wings

Without Wings

TH CaLexA No wings C

TH CaLexA No wings B TH CaLexA No wings

Update: Looking for the DA & OA neurons involved in phototactic flexibility

on Wednesday, October 7th, 2015 11:35

These are the newst results of my screen. There are some interesting candidates, but it’s still too soon to conclued something.


Update of the DA screening. New experiments are in red (25°C) and blue (32°C).



Experiment finished.


Looking for the DA & OA neurons involved in phototactic flexibility

on Monday, September 21st, 2015 2:41

After using TH-G4 and TDC2-G4 to prove that dopamine and octopamine are involved in phototactic switch after wing clipping, I decided to find which neurons in particular are playing this particular role. Therefore, I started a few small screens with the GAL4s and LexAs available for these groups of neurons. I crossed the drivers to shibire to assess their role. For every graph blue means RT, and red means 32°C.

TH subsets-GAL4


Dopaminergic neurons -GAL4

Da clusters



CS substrains in phototaxis

on Monday, August 3rd, 2015 2:58

I started testing the different CS substrains that we have here (CS from the paper https://f1000research.com/articles/3-176/v1 + CS Regensburg). I’ve decided to start with Benzer Paradigm. This is what I have so far.