I got mutant from Amita and will test them when out of quarantine. Then, we will have a clear answer about the compensatory mechanisms (the former mutant I tested was probably not null). There is also 2 other RNAi lines for PKC53e from the TriP project.

I am waiting for chrisi to be back and we will see what flies we need to order to bloomington in addition to these two. (There is also another RNAi against FoxP…)
The outcross of the RNAi is also running (3d cross, now).
We will then have 3 RNAi and one mutant, if the results are consistent, this would be enough to convince people that PKC53e is the PKC involved.