on Monday, June 19th, 2017 2:58 | by Axel Gorostiza
I am thinking of using optogenetics to study the neural substrates of photopreference. Here I present some controls that I have to do before starting. This is a T-Maze with blue light instead of with light.
on | by Axel Gorostiza
These are the last controls I needed in order to be sure everything wrong was gone. They are not the best, but I think they show some clear results.
Yellow: Room Temperature
on Monday, June 12th, 2017 2:53 | by Axel Gorostiza
To find the source of the problems I have seen in the T-Maze, I use lines from our stock and tested them in the T-Maze. This were reared at 25°C.
on Saturday, June 10th, 2017 4:21 | by Saloni Rose
on Thursday, June 1st, 2017 4:05 | by Saloni Rose
on Monday, May 29th, 2017 12:51 | by Saloni Rose
on Monday, May 22nd, 2017 12:17 | by Axel Gorostiza
on | by Saloni Rose
I want to continue on the Diazepam project and look if Serotonergic or Gabaergic circuits are involved in modulating photo-preference in flies. To begin with, I tried to replicate Lena’s experiments involving the individual T-maze. Each fly was introduced at the decision point of the T maze and once the fly moves towards either the light or the dark, it was pulled out of the T-maze using suction and reintroduced to the decision point (Each fly was tested ten times). In the first few days, I tried to standardise the apparatus and the light intensity. The results below indicate the choice index of normal and clipped WTB flies.
on Monday, April 24th, 2017 1:55 | by Axel Gorostiza
I am still testing different GAL4s in order to find out the dopamine clusters involved in photopreference shift. Here are some new results.
on Monday, April 3rd, 2017 1:56 | by Axel Gorostiza
Here I present new data on the GAL4s I have been testing. I also retested some previous ones. I had troubles with the shiTS/+ control, so the N has not chance since the last time.