Testing CaLexA

on Monday, October 30th, 2017 1:32 | by

Here I used the CaLexA tool with the elav driver, and reared the flies on constant darkness or under L-D cycles.

L-D Cycle


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OA and photopreference

on Monday, October 16th, 2017 2:14 | by

Here I tested two common OA drivers in photopreference. Although the results are not conclusive, there could be something there.

Yellow: 25°C

Green: 32°C


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TH activity in Photopreference

on Monday, October 2nd, 2017 11:12 | by

This is a new try to set up the CaLexA technique.

TH-CaLexA L-D 12:12 Without Wings

TH: Magenta

GFP: Green

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Sensory input and photopreference

on Monday, September 11th, 2017 11:41 | by

To begin studying the sensory input related to photopreference shift after wing clipping, I decided to test some propioception mutants. Here are the first results.

Green: Intact flies

Yellow: Wing-clipped flies

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Optogenetics (Blue and UV light in photopreference)

on Monday, September 4th, 2017 2:02 | by


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Optogenetics and photopreference

on Monday, July 24th, 2017 10:39 | by

I am still trying to set up the optogenetics tools for my photopreference assay. These are the results so far. I combined the result obtained with Chrimson in the second chromosome and in the X chromosome, because they were identical.

Blue: only blue light.

Red: Blue light + pulsed red light.



I think Chrimson is already activated under my blue light condition.


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Photopreference and optogenetics

on Monday, July 17th, 2017 2:57 | by

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Photopreference and Speed

on | by

This is something we talk last week. Here are the results for Speed and Stripe Deviation in the Buridan.


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Optogenetics in Photopreference (Pretests)

on Monday, July 10th, 2017 12:31 | by

On this opportunity, I changed the size of the tubes in order to see if that has a impact on the proportion of flies that ended in the elevator.


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Optogenetics in Photopreference

on Monday, July 3rd, 2017 1:26 | by

Here, I present you the results of using Blue light for photopreference and adding a pulsed Red light. These experiments are controls required for using Optogenetics to study Photopreference. I used the Blue light at different intensities, and calculated the Choice Index (CI) and the proportion of flies in each tube of the T-Maze (White: Bright, Black: Dark, Grey: Elevator).



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