Starting with optogenetics in photopreference

on Monday, June 19th, 2017 2:58 | by

I am thinking of using optogenetics to study the neural substrates of photopreference. Here I present some controls that I have to do before starting. This is a T-Maze with blue light instead of with light.

 

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Problems solved? (photopreference)

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These are the last controls I needed in order to be sure everything wrong was gone. They are not the best, but I think they show some clear results.

Yellow: Room Temperature

Green: 32°C

 

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Some controls for the photopreference Troubleshooting

on Monday, June 12th, 2017 2:53 | by

To find the source of the problems I have seen in the T-Maze, I use lines from our stock and tested them in the T-Maze. This were reared at 25°C.

 

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Diazepam Experiment (Conc= 15 mM)

on Saturday, June 10th, 2017 4:21 | by

N=10

 

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Update on the Diazepam Experiment

on Thursday, June 1st, 2017 4:05 | by

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Diazepam Experiment

on Monday, May 29th, 2017 12:51 | by

Pilot experiment(N=20)

 

Diazepam Experiment(N=10)

 

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DA clusters and Photopreference

on Monday, May 22nd, 2017 12:17 | by

Here is an update on the evaluation of the DA clusters in Photopreference.

 

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Preliminary testing of the Individual T maze

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I want to continue on the Diazepam project and look if Serotonergic or Gabaergic circuits are involved in modulating photo-preference in flies. To begin with, I tried to replicate Lena’s experiments involving the individual T-maze. Each fly was introduced at the decision point of the T maze and once the fly moves towards either the light or the dark, it was pulled out of the T-maze using suction and reintroduced to the decision point (Each fly was tested ten times). In the first few days, I tried to standardise the apparatus and the light intensity. The results below indicate the choice index of normal and clipped WTB flies.

 

 

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Update on DA & photopreference

on Monday, April 24th, 2017 1:55 | by

I am still testing different GAL4s in order to find out the dopamine clusters involved in photopreference shift. Here are some new results.

 

 

 

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Photopreference shift and DA (new and old GAL4s)

on Monday, April 3rd, 2017 1:56 | by

Here I present new data on the GAL4s I have been testing. I also retested some previous ones. I had troubles with the shiTS/+ control, so the N has not chance since the last time.

 

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