Since I finished the first round of the screen to find out the DA substrate of the photopreference, I was testing the candidate lines in a WTB background. I had some unexpected results with the controls, so now I’m continuing the experiments, while adjusting some things to understand the problem.

In this case, I reared and tested the flies as before (reared at 18°C, and tested at 25 & 32 °C in consecutive days). But, I also made a copy of those crosses and placed them at 25 during development. After that I moved them to 18°C until the experiment was done.

Update (25°C):