Update: Red light Joystick results

on Saturday, March 18th, 2023 1:28 | by

After the first full week of testing the three different fly lines with red light, we have analyzed the results. We are now able to provide a further update on our findings.

While the effect for the control groups seems to stay consistent with our previous findings, the effect for the positive control has weakened and is closer to the strength measured in previous experiments. We utilized the norpAP24;Gr28bd+TrpA1>Chrimson line for both our positive and negative controls.

Furthermore, the experimental group, which previously favored red light in the post-test, showed a more varied and inconclusive profile. We will need to conduct further testing to determine whether the previously observed effect was simply caused by the small sample size.

Another important discovery was the necessity of feeding the flies sugar water 5-10 minutes before the experiment in order to maintain their activity levels. This way the flies will be identified as a valid data point by the R-script and will ensure more and more accurate data. It is important to note that we will implement this feeding process in all future experiments to ensure consistency and accuracy of the data collected.

Red light Joystick Results

on Tuesday, March 14th, 2023 8:57 | by

The box plots show the performance indices (PIs) of each fly as one dot for each minute of the test and for the pre- and post-test periods. Values between 1 and -1 indicate preference for or avoidance of the light.
The light used is red and has an intensity of 400 to 470 lux, the LEDs have a voltage of 7.5 volts. The control flies observed seem to show a stronger effect than in previous tests, which could be due to the use of a thinner fishing line (0.6 mm), which is easier to place correctly in the clamp of the joystick machine.
For the negative and positive control we used the line norpAP24;Gr28bd+TrpA1>Chrimson.

Red Joystick Results

on Friday, September 23rd, 2022 6:55 | by

Experiments of all strains completed.

Yellow Joystick Results

on Friday, September 23rd, 2022 6:50 | by

Experiments for all strains completed.

Red Joystick Results

on Monday, September 19th, 2022 9:40 | by

Red joystick results of all strains. By Enes, Aslıhan, Vivi.

Yellow Joystick Results

on Sunday, September 18th, 2022 6:37 | by

Yellow joystick results of all strains. By Enes, Aslıhan, Vivi.

Comparison of T-maze and Joystick Results

on Sunday, September 18th, 2022 11:26 | by

Two different ways of presentation for red and yellow light. With the term Joystick here, the data collected for the last training period of each line are represented. Results obtained from Enes, Aslihan and Vivi.

Joystick Red Results of All Strains; Enes Aslıhan Vivi

on Saturday, September 17th, 2022 12:38 | by

These are results of red joystick experiments of all strains.

Joystick Yellow Results of All Strains; Enes Aslıhan Vivi

on Friday, September 16th, 2022 11:59 | by

These are results of yellow joysticks experiments of all strains.

Joystick results of NOATR, ATR Control and 13.0273, SS5699 for RED light Enes Aslıhan Vivi

on Wednesday, September 14th, 2022 11:15 | by

Joystick results of NOATR, ATR Control and ATR 013.0273, ATR SS5699 for RED light.

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