Weitian Sun

Summary of results of master thesis

Self-learning of Rover/sitter from flight simulator

Long-term memroy from self-learning

Self-learning training/tests from transgeneic flies

Chrimson/Gr28bd lines tests in yellow light (580nm)

new TrpA/chrimson self-learning tests

NorpA; Chrimson/Gr28 (N=12, ATR)

NorpA; Chrimson/Gr28 (N012, No ATR)

NorpA;Chrimson/+ (N=11, ATR)

Normal vision on platform test

Self learning test and long-term memory test of TrpA1 from optogenetic training

TrpA1 self learning test, N= 47

TrpA1 long term memory, N= 39

Wtb long term memory (previous on fly simulator), N= 16

self-learning update

N= 31

update on self learning test comparsion

30 from each lines, no statistially difference among them. I will keeping testing on flight simultor before transgenic flies hatch.

Correct the longterm memory results

longterm memory test

N= 30, p-value = 0.66

Rescue from forS in visual memory defects:

adult-limit for expression in fan-shape body (c205-Gal80ts)

increase for expression in ellipsoid body (c819-Gal80ts)

R,S,WTB updates, and methods to inhibate PKG

Rover,N= 36

Sitter, N= 28

WTB, N= 13

PKG affects K channels

Two ways to inhibitatePKG:

1

UAS-Gal4, RNAi

UAS-GCaMP3/OK6-GAL4; UAS-for RNAi/+

UAS-GCaMP3/OK6-GAL4; UAS-for RNAi/ UAS-for RNAi

2

PKG inhibitors:

1. KT 5823(10mM in DMSO)
2. (irrversible)