Testing CaLexA
Here I used the CaLexA tool with the elav driver, and reared the flies on constant darkness or under L-D cycles.
L-D Cycle
D-D
OA and photopreference
Here I tested two common OA drivers in photopreference. Although the results are not conclusive, there could be something there.
Yellow: 25°C
Green: 32°C
TH activity in Photopreference
This is a new try to set up the CaLexA technique.
TH-CaLexA L-D 12:12 Without Wings
TH: Magenta
GFP: Green
Sensory input and photopreference
To begin studying the sensory input related to photopreference shift after wing clipping, I decided to test some propioception mutants. Here are the first results.
Green: Intact flies
Yellow: Wing-clipped flies
Optogenetics and photopreference
I am still trying to set up the optogenetics tools for my photopreference assay. These are the results so far. I combined the result obtained with Chrimson in the second chromosome and in the X chromosome, because they were identical.
Blue: only blue light.
Red: Blue light + pulsed red light.
I think Chrimson is already activated under my blue light condition.
Photopreference and Speed
This is something we talk last week. Here are the results for Speed and Stripe Deviation in the Buridan.
Optogenetics in Photopreference (Pretests)
On this opportunity, I changed the size of the tubes in order to see if that has a impact on the proportion of flies that ended in the elevator.
