self-learning experiments: no phenotype with d42Gal4,chaGal80?
on Thursday, October 11th, 2012 6:33 | by Julien Colomb
[pdf lab.brembs.net/wp/wp-content/uploads/2012/10/tG80_PKCi_c380G_subset.pdf 500 600]
n=6 but high self learning score for UAS-GFP; chaGal4,chaGal80 x tubGal80ts; UASPKCi (that I wanted to use as a positive control).
| Possible cause | putative solution | |
| UASPKCi flies are not clean | got new flies from the stock | |
| start new crosses to redo the line ? | ||
| will test d42Gal4 as a driver again (waiting for males) | ||
|
crosses to get d42Gal4,chaGal80ts sans la construction GFP | |
| Ask for fly and forsee new crosses for c380Gal4 | ||
| Biology | I will think about it when all other possibilities will be tested for. | |
next step is to test a real positive control again (d42Gal4 or elavGal4 if I do not have any d42Gal4 males tomorrow), along with the d42Gal4,chaGal80.
Category: operant self-learning
Definitely do the orginal d42 cross, but also keep in mind that the controls didn’t learn and with single digit flies, the score can be spurious: maybe with 20 flies the score will be down to where you expected it to be…