on Thursday, October 11th, 2012 6:33 | by Julien Colomb
[pdf lab.brembs.net/wp/wp-content/uploads/2012/10/tG80_PKCi_c380G_subset.pdf 500 600]
n=6 but high self learning score for UAS-GFP; chaGal4,chaGal80 x tubGal80ts; UASPKCi (that I wanted to use as a positive control).
|Possible cause||putative solution|
|UASPKCi flies are not clean||got new flies from the stock|
|start new crosses to redo the line ?|
|will test d42Gal4 as a driver again (waiting for males)|
|crosses to get d42Gal4,chaGal80ts sans la construction GFP|
|Ask for fly and forsee new crosses for c380Gal4|
|Biology||I will think about it when all other possibilities will be tested for.|
next step is to test a real positive control again (d42Gal4 or elavGal4 if I do not have any d42Gal4 males tomorrow), along with the d42Gal4,chaGal80.
Category: operant self-learning