As a control for my T-Maze experiments I decided to test if treated flies were able to fly at the same conditions they were tested in the T-Maze (32°C).

As in the T-Maze experiments, I put the flies 15 min at 32°C before testing them at the same temperature.

The dashed line at 15 min indicates when a T-Maze experiment would have ended.

All flies tested flew almost twice the time required to perform a T-Maze experiment.