on Monday, October 26th, 2015 2:49 | by Bianca Birk
I continue testing the tbh-Gal4 subgroups and tbh-lexA.
Figure 1 shows the newest results of tbh-lexA (n=6)
Figure 2 shows the newest results of tbh-Gal4 (tbh48331, tbh46970 n=3, tbh39942 n=1, rest with a n of 2)
I practiced the dissections of brains for stainings. So I fixed the flies for one hour in PFA 4% , then I dissected the brains, washed them a few times with PBST and after it I mount them with vectashield.
Figure 3 shows a picture of a brain of a Cha-Gal80;TH-Gal4 x GFP (nucl.) flie.
Category: wing clipping