T-Maze experiments : screen results as on 25-06-2018

Yellow 1 (Positive Control):  Gr28bd-G4, TrpA1-G4

Parameters:
Light: intensity (500 Lux side, 1000 Lux bottom)

frequency = 20Hz

Delay = 1 ms

Duration = 9.9 ms

volts = 6.4

Initial screen results

Yellow 1 (Positive Control):  Gr28bd-G4, TrpA1-G4

Light: intensity (500 Lux side, 1000 Lux bottom), frequency (20Hz)

Tmaze experiments : Test for Blindness and Comparison with positive control

Comparison between White 1 ( Control (NorpA- UAS Chrimson)) and Yellow 1 (Positive Control ( Gr28bd-G4, TrpA1-G4).

To test whether the flies are really blind, and there is no problem with the NorpA part of the construct, we compare with NorpA,UAS GTACR1 ; NorpA,UAS GTACR2 ; and another stock of NorpA-UAS-Chrimson.

Weighted mean is calculated by multiplying the weights (total number of flies in that experiment/total number of flies in all the experiments) with the PI for that experiment, and taking the sum thereafter for all the experiments.

Tmaze experiments initial results

the first graph shows Bar plot of Mean and standard deviation of PIs for Genetic control (n=9) and Positive control (n=5).
The second graph shows weighted mean and weighted standard deviation of the same.

Weighted mean is calculated by multiplying the weights (total number of flies in that experiment/total number of flies in all the experiments) with the PI for that experiment, and taking the sum thereafter for all the experiments.

Optogenetics – My results vs. Lena’s results

Last week I continued the experiments for the optogenetics. These are my results:

and these are Lena’s results:

Compared to Lena’s results, my results are a bit too positive.

Optogenetics (Chrimson) with the Tmaze

Last week, I started with the optogenetic experiment. Two days before the experiment,  I put male flies into little vials (lightproof) containing standard food medium and I added a dab of yeast mixed with eihter ethanol (for control group) or ATR (= all-trans-retinal) (for experiment group).

These are the experimental conditions:

~ 1600 lx; 20 Hz; 10 ms puls width; short tubes (transparent tube with red light and an opaque dark tube); 30 sec in the elevator; 1 min testing; 4 hours between the first testing and the repetition;