T- maze experiments were done with Norpa/Crimson x Gr28bd/TrpA1 lines without ATR (n=18) and with ATR (n=22) and 5 different groups (THD’-GAL4xNorpA/Crimson with and without ATR, THD1-GAL4xNorpA/Crimson with and without ATR and W[1118]x NorpA/Crimson). Group1 (n=4) Group2(n=4) Group3(n=3) Group4 (n=3) Group5(n=1)We don’t know that the which lines belong to which groups to make the process more reliable. The graph shows the PI numbers according to the T-maze experiments that we have done.

At a set frequence of 60Hz the FPGA overshoots the measurement with .41hz. For a 25 minute experiment as above this means an extra 10.25 seconds.

T-maze experiments were done with Norpa/Crimson x Gr28bd/TrpA1 lines without ATR (n=14) and with ATR (n=15) and. The graph shows the PI numbers according to the T-maze experiments that we have done.

Norpa/Crimson x Gr28bd/TrpA1 lines waited in vials which two of them include ATR and other two not. After doing T- maze experiment (at yellow light) their wings were clipped and next day Buridan test was done with them. This graph shows the stripe deviation of experimented flies. ATR dark(n=3), ATRlight(n=3),NoATRdark(n=4),NoATRlight(n=5). The result shows that experimented flies could not see and their motions were random.

The histogram plotting the time differences in the data collection from the FPGA showed that an absolute majority of the data was collected with the set frequency, 60hz. There were a few outliers from these 16ms and to find out if these spikes occurred with any kind of pattern I plotted the time differences over time. While the increases seems to appear seemingly random, most time diff increases also seem to be accompanied with a time diff decrease.

Periods without laser has more data collection points

The optomotor stimulus consists of a striped arena with panoramic vertical stripes rotating horizontally around the vertical, or yaw axis. Depending on the spatial wavelenght (or spatial frequency, SF) the fly’s optomotor response (OR) is changing supposedly to reach a maximum between 20-40°
(with a pattern motion of 0° ) . As the panoramic stripes are rotating a tethered fly will actively steer in the direction of the motion. After its maximum the OR gradually decreases with an increasing spatial frequency to finally reach 0 around 9.6-6.3°. A tendency to negative/reversed OR has been observed as the SF reaches 6.3°.

This experiment has been shown before in 1976 by E. Buchner, Elementary movement detectors in an insect visual system. Bioi Cybern 24:85- 101, Figure 8 page 93. Attaching Figure 8 as a reference.