FoxPB-Gal4 + p-smad (motorneurons)
The reaction is not optimal, but it looks like there are some double positive cells, and this indicate that some neurons could be motorneurons. To identify a % more and better reactions should be done
Entire larva:
FoxP-KO
FoxP-KO: the homozigous FoxP-KO (with NO Gal4 and NO GFP) had a green signal, it means that the dsRed gives problems. I thus removed it. Also, in really nice dissections, I don´t see the phenotype I was seeing before. The brain looks fairly normal. it could than be that i wasn´t disecting well enough
Hybridoma (Fusion) 1st screen
This picture shows all of the positive clones in the ELISA from the 1st screen
While this picture shows just the clones that have a high response for the peptide the mice where injected with, a low response for any other random peptide, and furthermore a low response for BSA
FoxPB adult cell count
I add another animal to the count (so now n=5) and the standard deviation increased. Since I used a very young fly it could be that the cell number is age dependent. But since it can be also individual differences, after San Diego I will make an age related analysis
FoxP-KO or WTB?
Here´s a picture of a WTB fly and a foxP-KO-homozigous. I dissected all together and I did not know which one was which. But I manage to recognize them, which means that they are well recognizable. Just to be sure i will do that another time. (Also in order to have better dissections).
The distance between the two “hemispheres” in the WTB has an average of 5,4 um (n=3)
The distance between the two “hemispheres” in the KO has an average of 32,2 um (n=2)
The length of the protocerebral bridge in the WTB has an average of 95,7 um (n=3)
The the protocerebral bridge in the KO has an average of 140,3 um (n=2)
63x picture of 2 of the FoxPB-Gal4 positive clusters
The dorsal cluster neurons are really evident and with a typical organization
From Hassam et al., 2000
Protocerebral bridge in FoxP-KO flies
The more brains I see, the more I am convinced that the problem is not about my dissection
MB in FoxPB-Gal4 with GFP on the 2nd chromosome (homozigous)
this result is still to be verified by deleting the red fluorescent eyes to the flies
Counting of FoxP-isoB-Gal4 positive cells
Counting of FoxP-isoB-Gal4 positive cells in adult and larvae brains (no VNC). Five brains counted for each age
