Ottavia Palazzo

ELAV-Gal4;Tub-Gal80: Temperature switch at embryo stage

We phenocopied the results of the ELAV-Gal4 cross.

Now we are waiting for the results of the Temperature switch at the second instar larvae stage

New figure?

August

• Halfway in analyzing the Buridan data altogether (not in two batches)
• Maxiprepped plasmid for making FoxP protein at the klinikum
• Writing the thesis a little bit
• bought Fas-II antibody

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• data 1): FoxP-iB Heterozygous/Homozygous comparison with Stinger-GFP. This time i was cautious with everything: fly all the same age and sex, same larvae density, same number of copies of GFP

I can not detect any difference between homozygous and heterozygous mutants. I also counted cells in IMARIS and I detected no difference in number or distribution.

• data 2): FoxP-iB Heterozygous/Homozygous comparison with CD8-GFP. This time i was cautious with everything: fly all the same age and sex, same larvae density, same number of copies of GFP

I can see some differences but i do not know how to quantify/explain them. Also I am not sure if it is a problem of dissection/mounting. I am not convinced.

• data 3): nc82 staining on homo/hetero FoxP-iB. I do not see differences

Problem: I haven’t managed yet to make the FasII antibody work:

I have used a 1:10 concentration of primary antibody for one night and a 1:100 concentration of secondary antibody for 4 hours. I will ask someone

In the picture I have increased the gain a lot just to be sure. I could not see anything

Pupae TS increased N

Tdc2-Gal4 + FoxP-LexA in VNC

C380-increased N

Last week

1. Crossed the flies for the course
2. Almost finished the Buridan for C380
3. Worked on the manuscript and supplemetary figures
4. Done the FoxP reaction on induced embryo again because I was afraid after the different results Andi had last time

ok107-increased N

qPCR-Joel

Last week

1. Made crosses for pupae TS
2. Made cross for Tdc2-Gal4 and FoxP-LexA to see the VNC (I already had last week the pictures of the CB)
3. Made crosses for ok107
4. Made crosses for C380

After all those crosses will hatch I will have finished with the Buridan for now.