For the stressor ‘cold’ the flies were kept on 18°C for 48h on ATR.

stressor: cold (18°C for 48h)

POS_CTRL: control group

Gr28bd+TrpA1(cold): experimental group

For the stressor ‘crowding’ ca. 60-65 flies were kept in one vial for 48h on ATR.

The effect of crowding was created by the volume of air available to the flies in the vial. This was altered via change in the height of the cotton plug in the vial. The control group had the cotton plug at the usual height of ca. 60mm above the food in the vial and the experimental group only 20mm.

stressor: crowding (maintained in smaller volume for 48h on ATR)

POS_CTRL: control group

Gr28bd+TrpA1(Crow): experimental group