Crosses to check the TARGET flies

on Tuesday, December 4th, 2012 12:49 | by

Since we have problems with Madeleine’s project, I will make crosses to test that the flies are ok before using them for PKC RNAi experiments.

the putative elavGal$;tubGal80ts flies will be crossed to w; AKAP/Cyo flies. Then the son ElavGal4/Y, tubGal80ts/+ will be crossed to w.

Only non Cyo male flies should get white eyes, if not, the stock is not clean.


In parallel, I will also start crossing nsybGal4 into the tubGal80ts line (2 to 4 crosses necessary depending on the eye color of the heterozygotes).