Control Experiments for Driver Line Expression
on Monday, February 9th, 2026 12:44 | by Ipek Subay
SS45779 – Targeting b3 MN, iii3 MN, hg1 MN
Our line:
Janelia image:
IS69306 – b1 mn driver line
Janelia image:
Retro-Tango control crossed with GF-Gal4 (BDSC: 79602), presynaptic neurons in magenta, giant fiber neurons in cyan and background staining in gray
Retro-Tango x GF-Gal4 from Sorkac et. al (2023)
rut and rad expression in b1 and b3 motor neurons, I looked for cells express 6 genes (rut, rad, FoxP, VGlut, AD TF, DBD TF) at the same time above certain threshold:
| Driver Line | AD TF | DBD TF | Targeted cells | Cells express all 6 genes at more than 0 | Cells express all 6 genes at more than 1 fold | Cells express all 6 genes at more than 2 fol |
| SS40980 | CG12680 | Cep104 (CG10137) | b1 MN, iii1 MN, iii3 MN | 0 | 0 | 0 |
| SS45779 | Ubx | ab | b3 MN, iii3 MN, hg1 MN | 158 | 0 | 0 |
| SS47160 | Ubx | hry | b2 MN, b3 MN, iii4 MN, hg1 MN, hg3 MN | 1 | 0 | 0 |
| SS48311 | Ubx | NetB | b3 MN, hg1 MN, XBI002, WBI009 | 78 | 44 | 2 |
| SS49806 | ??? | tim | b3 MN, hg1 MN, HBI017 | – | – | – |
| SS52405 | Ubx | CG4168 | b3 MN, i2 MN, hg3 MN | 46 | 25 | 1 |
| IS69306 | CG14388 (CG31345) | Awh | b1 driver | 0 | 0 | 0 |
| SS98650 | ct | Ubx | b3 driver | 76 | 39 | 0 |
| IS38497 | beat-IIIb | bab1 | b1&b3 | 34 | 15 | 1 |
Category: Anatomy, Motor neurons, retro-tango | No Comments