Ottavia Palazzo

Supplementary figure S1 and S2

Waiting for crosses to hatch

1. ato-Gal4 x conditional KO —> Removing FoxP in Dorsal Cluster Neurons for Buridan
2. cmpy-Gal4 x conditional KO —> Removing FoxP in Protocerebral Bridge for Buridan
3. temperature switch at embryo stage but shorter time —> for replicating the KO phenotype
4. LexA + Gal4 together to analyze larval brain (I have always considered larvae except for this experiment, so I am doing it to be more complete)

Furthermore, for not loosing time this week I did:

1. Leipzig presentation
2. Wrote a bit of results for paper/thesis

Graphs new look

LexA construct qPCR

Additional ELAV-Gal4>UAS-gRNA control

Last week

1. extracted RNA and done cDNA from it from WTB flies and Hetero flies (Homo were too few). Doing the qPCR with those this week
2. Inserted GFP in homozygosis in FoxP-KO flies
3. Crossed Elav-Gal4 x UAS-gRNA-FoxP and Elav-Gal4;Tub-Gal80ts x UAS-gRNA-FoxP (left at 30 degrees) as controls for buridan
4. Crossed protocerebral bridge line for buridan
5. Finished the presentation for thursday
6. Writing material and methods for the thesis

Het-Homo OL

Protocerebral bridge lines

C380-Gal4 on conditional KO

CD8-GFP on Hom and Het on DCN

Quiz: which is Het and which is Hom? Which differences can you see?