Waiting for crosses to hatch
- ato-Gal4 x conditional KO —> Removing FoxP in Dorsal Cluster Neurons for Buridan
- cmpy-Gal4 x conditional KO —> Removing FoxP in Protocerebral Bridge for Buridan
- temperature switch at embryo stage but shorter time —> for replicating the KO phenotype
- LexA + Gal4 together to analyze larval brain (I have always considered larvae except for this experiment, so I am doing it to be more complete)
Furthermore, for not loosing time this week I did:
- Leipzig presentation
- Wrote a bit of results for paper/thesis
LexA construct qPCR

Second graph: FoxP-LexA
Last week
- extracted RNA and done cDNA from it from WTB flies and Hetero flies (Homo were too few). Doing the qPCR with those this week
- Inserted GFP in homozygosis in FoxP-KO flies
- Crossed Elav-Gal4 x UAS-gRNA-FoxP and Elav-Gal4;Tub-Gal80ts x UAS-gRNA-FoxP (left at 30 degrees) as controls for buridan
- Crossed protocerebral bridge line for buridan
- Finished the presentation for thursday
- Writing material and methods for the thesis
Het-Homo OL

Protocerebral bridge lines
