Ottavia Palazzo

View Profile

Supplementary figure S1 and S2

on Thursday, February 27th, 2020 11:13
Category: Foxp | No Comments

Waiting for crosses to hatch

on Wednesday, February 26th, 2020 1:46
  1. ato-Gal4 x conditional KO —> Removing FoxP in Dorsal Cluster Neurons for Buridan
  2. cmpy-Gal4 x conditional KO —> Removing FoxP in Protocerebral Bridge for Buridan
  3. temperature switch at embryo stage but shorter time —> for replicating the KO phenotype
  4. LexA + Gal4 together to analyze larval brain (I have always considered larvae except for this experiment, so I am doing it to be more complete)

Furthermore, for not loosing time this week I did:

  1. Leipzig presentation
  2. Wrote a bit of results for paper/thesis

Graphs new look

on Wednesday, February 19th, 2020 11:17
Category: Foxp | No Comments

LexA construct qPCR

on Wednesday, February 12th, 2020 3:35
First graph: FoxP-iB-Gal4
Second graph: FoxP-LexA
Category: Foxp | No Comments

Additional ELAV-Gal4>UAS-gRNA control

on Thursday, February 6th, 2020 5:23

Last week

on Friday, January 31st, 2020 3:50
  1. extracted RNA and done cDNA from it from WTB flies and Hetero flies (Homo were too few). Doing the qPCR with those this week
  2. Inserted GFP in homozygosis in FoxP-KO flies
  3. Crossed Elav-Gal4 x UAS-gRNA-FoxP and Elav-Gal4;Tub-Gal80ts x UAS-gRNA-FoxP (left at 30 degrees) as controls for buridan
  4. Crossed protocerebral bridge line for buridan
  5. Finished the presentation for thursday
  6. Writing material and methods for the thesis
Category: Foxp | No Comments

Het-Homo OL

on Friday, January 24th, 2020 12:35
Homo, Hetero

Protocerebral bridge lines

007Y-Gal4, GMR55G08-GAL4

C380-Gal4 on conditional KO

on Wednesday, January 22nd, 2020 2:46

CD8-GFP on Hom and Het on DCN

on Monday, January 13th, 2020 1:24

Quiz: which is Het and which is Hom? Which differences can you see?

Category: Foxp | No Comments