Results of salt (1.5 M) avoidance tests for the second crossing under red light

A: dicer2; SNPFR-RNAi × TH-Gal4
B: w1118 × TH-Gal4
C: w1118 × dicer2; SNPFR-RNAi
Larvae were placed in the center of an agar plate with plain agar on one side and salt-containing agar (1.5 M NaCl) on the other. After 3 minutes, larval distribution was quantified using a preference index under red light. All three groups showed significant salt avoidance and no significant differences were observed between the groups.
Results of salt (1.5 M) avoidance tests for the first crossing under red light

A: w1118 × dicer2; SNPFR-RNAi
B: w1118 × dicer2; H24-Gal4
C: dicer2; SNPFR-RNAi × dicer2; H24-Gal4
Larvae were placed in the center of an agar plate with plain agar on one side and salt-containing agar (1.5 M NaCl) on the other. After 3 minutes, larval distribution was quantified using a preference index under red light. All three groups showed significant salt avoidance, but no significant differences were observed between the groups.
First results of salt (1.5 M) avoidance tests in groups A, B, and C under red light

Drosophila larvae from three groups (A, B, and C) were placed in the center of an agar plate containing plain agar on one side and salt-containing agar (1.5 M) on the other. After 3 minutes, larval distribution was quantified using a preference index under red light. All three groups showed a significant avoidance of salt. No significant differences in salt avoidance were observed between the groups.
Salt avoidance test in control larvae (WTB) under red light and blue light (combined data)

Larvae showed a significant avoidance of salt under both light conditions. When data from both weeks were combined, no significant difference in avoidance was observed between blue and red light.
Salt avoidance test in control larvae (WTB) under red light and blue light

WTB Drosophila larvae were placed in the center of an agar plate with plain agar on one side and salt-containing agar on the other. After 3 minutes, larval distribution was quantified as a preference index under red and blue light.
Larvae showed a significant avoidance of salt under both light conditions. Avoidance was significantly stronger under blue light than under red light.
Salt avoidance test in control larvae (WTB) under red light and blue light

WTB Drosophila larvae were placed in the center of an agar plate containing plain agar on one half and agar supplemented with salt on the other half.
Larval distribution after 3 minutes was quantified as a preference index under red and blue light conditions.