1.New fusion protein construct

Goal_1: Fuse aPKC N-terminally to TurboID linker sequence to prevent steric hindrance of the C-terminal kinase

Goal_2: Establish Sco/Cyo; aPKC-TurboID/aPKC-TurboID lines

Crossed Sco/Cyo; D3/TM3 with ;;vasphi31,attp86FB lines, collected red eyed offspring and recrossed with double balancer

Goal_3: Confirm aPKC expression via larval survival essay

2. Yaw torque experiments with UAS-cas9;nsyb-GS_Gal4/CadN-gRNA

2.1 Averaged AI at start

2.2 Averaged AI at end

2.3 PI per period_box plot