On Wednesday I tested the “heatshock” group, using light (with the same intensity as the aversive stimulus in the joystick experiment: ~800lux) for 5 minutes before testing, to stress the flies.

Stressor: heat (5min; 7.4V; ~800lux)

Gr28bd+TrpA1(Co): control group

Gr28bd+TrpA1: experimental group

 
On Friday I tested flies who were maintained in a cold environment (18°C) during the ATR treatment (2 days).

Stressor: cold (18°C; 48h)

Gr28bd+TrpA1(Co): Control group

Gr28bd+TrpA1: Experimental group