Recent results revealed problems with our negative control (Gr28bd-Gal4; TrpA1-Gal4+ EtOH) treated with EtOH. As the food for these flies was not substituted with ATR but only with Ethanol (ATR is dissolved in EtOH), the Chrimson channel should be non-functional in this group and the flies should therefore not avoid light. However, we observed negative PIs for this group. Since some activation of the Chrimson channel could be possible even without ATR, I tried to lower the used light intensity to values where the positve control would still avoid light, but the intensity would be too low for the Chrimson channel to activate when no ATR is present.

It seems that 650 Lux might still be too high. Therefore I will conduct another trial with 500 Lux.