LexA+Gal4 larvae

on Friday, March 13th, 2020 3:30 | by Ottavia Palazzo

I tried the fast way but is not usable. Because without doing an additional crossing I have only GFP and RFP in heterozygosis. which gives a very weak signal. Furthermore I have to remove (flox) the dsRED that is there from the LexA insertion. Otherwise in the larva I see some cells that are red but that are due to the dsRED and not to the RFP. Which means other crossings. In total, to have this picture I need 5 crosses.
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Category: Foxp

2 responses to “LexA+Gal4 larvae”

  1. Björn Brembs says:
    March 16, 2020 at 08:58

    That’s a lot of crosses. But I think it is necessary, if you want to document expression thoroughly.

    Reply
    • Ottavia Palazzo says:
      March 16, 2020 at 10:13

      Yes it´s a lot but it´s not so much time in total.. it´s just remembering to cross :) I think I would like to have this picture, it makes everything more complete

      Reply

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