Male and female groups after heat-shock (HS) and non-stress (n.S.) conditions. HSP70 was detected using a rat anti-HSP70 primary antibody and a donkey anti-rat secondary antibody with fluorescence detection at 700 nm (red). Actin was used as loading control (intensity slightly edited) and detected using a rabbit anti-actin primary antibody and a goat anti-rabbit secondary antibody at 800 nm (green). HSP70 signal intensity was quantified and normalized to the corresponding actin signal.

Increase of HSP70 expression after heatshock. Difference in increase between sexes is not significant (t-test, p-value = 0.3).