on Wednesday, October 7th, 2015 11:35 | by Axel Gorostiza
Update of the DA screening. New experiments are in red (25°C) and blue (32°C).
on Monday, September 21st, 2015 2:41 | by Axel Gorostiza
After using TH-G4 and TDC2-G4 to prove that dopamine and octopamine are involved in phototactic switch after wing clipping, I decided to find which neurons in particular are playing this particular role. Therefore, I started a few small screens with the GAL4s and LexAs available for these groups of neurons. I crossed the drivers to shibire to assess their role. For every graph blue means RT, and red means 32°C.
Dopaminergic neurons -GAL4
on Wednesday, April 3rd, 2013 9:14 | by Lidia Castro
I have started doing experiments with the transparent T-maze, but I have some problems. The results are really weird: I have obtained negative phototaxis for both flies with intact and clipped wings (phototaxis is more negative for flies with intact wings????).
I did only 9 experiments, because I don’t really know how to run them and I need advice. I am going to explain how I was doing this experiments until now:
– First of all, I put the flies in the entrance tube of the T-maze (upper part) and I cover the T-maze with a black clothing (I want the flies to be adapted to the darkness, like in my last experiments with the opaque T-maze). They are in this situation for 10min.
– After that I push the flies into the elevator. I don’t know if I should let them stay there for 1 minute (like I was doing before with the opaque T-maze, or push them to the bottom of the T-maze to let them make their decision between darkness or light without an adaptation time.
I think that if they stay in the elevator for 1 minute, they will be again under light conditions, and that’s not good for the experiment. I could cover again the T-maze with the black clothing during this minute (that’s what I was doing until now, but with bad results).
– Then I let the flies decide for 30 seconds as always.
So if you have any suggestions, I will be glad to hear them :)
on Thursday, November 15th, 2012 2:39 | by Lidia Castro
Yesterday, we cut the wings of half of the experimental flies.
We let them get over for 2 hours, and after that I repeated the same experiment we did last day, letting them choose between darkness and light.
There wasn’t a big difference between flies with wings and flies without wings, but the experiment shows flies without wings have less light preference than the other ones.