Role of dopaminergic neurons in operant behaviour

on Friday, July 27th, 2018 3:54 | by

Positive Control: Gr28bd-G4, TrpA1-G4

Parameters: Light: intensity (500 Lux side, 1000 Lux bottom); frequency = 20Hz; Delay = 1 ms; Duration = 9.9 ms; volts = 6.4

Red lines: completed

mb025b: not selected against tubby

The Tmaze Experiments : Screen results as on 22-7-18

on Sunday, July 22nd, 2018 6:41 | by

 

Yellow 1 (Positive Control): Gr28bd-G4, TrpA1-G4

Parameters: Light: intensity (500 Lux side, 1000 Lux bottom); frequency = 20Hz; Delay = 1 ms; Duration = 9.9 ms; volts = 6.4

The T-Maze experiments : Middle analysis and Correlation plot

on Saturday, July 14th, 2018 12:06 | by

The mean ratio of the flies that stay in the middle during the experiments.

Correlation plot between the mean ratio of the flies that stay in the middle versus the Weighted PIs

Slope = 0.0053
Intercept = 0.240
R square value = -0.03834

contrary to the expectations, there seems to be no correlation .

T-Maze experiments : screen results as on 02-07-2018

on Monday, July 2nd, 2018 2:09 | by

Yellow 1 (Positive Control): Gr28bd-G4, TrpA1-G4

Parameters:
Light: intensity (500 Lux side, 1000 Lux bottom)

frequency = 20Hz

Delay = 1 ms

Duration = 9.9 ms

volts = 6.4

Tmaze Exploratory analysis : Middle Flies

on | by

plotting the mean ratio of flies which stay in the middle during experiment.

 

Yellow 1 (Positive Control):  Gr28bd-G4, TrpA1-G4

Parameters:
Light: intensity (500 Lux side, 1000 Lux bottom)

frequency = 20Hz

Delay = 1 ms

Duration = 9.9 ms

volts = 6.4

T-Maze experiments : screen results as on 25-06-2018

on Monday, June 25th, 2018 1:13 | by

Yellow 1 (Positive Control):  Gr28bd-G4, TrpA1-G4

Parameters:
Light: intensity (500 Lux side, 1000 Lux bottom)

frequency = 20Hz

Delay = 1 ms

Duration = 9.9 ms

volts = 6.4

Tmaze experiments : Test for Blindness and Comparison with positive control

on Monday, June 11th, 2018 12:45 | by

Comparison between White 1 ( Control (NorpA- UAS Chrimson)) and Yellow 1 (Positive Control ( Gr28bd-G4, TrpA1-G4).

To test whether the flies are really blind, and there is no problem with the NorpA part of the construct, we compare with NorpA,UAS GTACR1 ; NorpA,UAS GTACR2 ; and another stock of NorpA-UAS-Chrimson.

 

Weighted mean is calculated by multiplying the weights (total number of flies in that experiment/total number of flies in all the experiments) with the PI for that experiment, and taking the sum thereafter for all the experiments.

Update: Looking for the DA & OA neurons involved in phototactic flexibility

on Wednesday, October 7th, 2015 11:35 | by

These are the newst results of my screen. There are some interesting candidates, but it’s still too soon to conclued something.
THs

 

Update of the DA screening. New experiments are in red (25°C) and blue (32°C).

 

THs

Experiment finished.

THs

Looking for the DA & OA neurons involved in phototactic flexibility

on Monday, September 21st, 2015 2:41 | by

After using TH-G4 and TDC2-G4 to prove that dopamine and octopamine are involved in phototactic switch after wing clipping, I decided to find which neurons in particular are playing this particular role. Therefore, I started a few small screens with the GAL4s and LexAs available for these groups of neurons. I crossed the drivers to shibire to assess their role. For every graph blue means RT, and red means 32°C.

TH subsets-GAL4

THs

Dopaminergic neurons -GAL4

Da clusters

 

TbH

New experiments

on Monday, December 10th, 2012 3:01 | by

I was trying to increase the differences between flies with clipped and intact wings, putting a paper covering the LEDs.

Results are not as good as I expected. The differences are lower than experiments done without this paper between LEDs and flies. I did experiments only with the big white LEDs (the ones which showed better results).