The Tmaze Experiments : Screen results as on 22-7-18

on Sunday, July 22nd, 2018 6:41 | by

 

Yellow 1 (Positive Control): Gr28bd-G4, TrpA1-G4

Parameters: Light: intensity (500 Lux side, 1000 Lux bottom); frequency = 20Hz; Delay = 1 ms; Duration = 9.9 ms; volts = 6.4

T-Maze experiments : screen results as on 16-07-2018

on Monday, July 16th, 2018 1:32 | by

Results of the T-maze screen analysis, both individual and combined.

Yellow 1 (Positive Control): Gr28bd-G4, TrpA1-G4

Parameters: Light: intensity (500 Lux side, 1000 Lux bottom); frequency = 20Hz; Delay = 1 ms; Duration = 9.9 ms; volts = 6.4

The T-Maze experiments : Middle analysis and Correlation plot

on Saturday, July 14th, 2018 12:06 | by

The mean ratio of the flies that stay in the middle during the experiments.

Correlation plot between the mean ratio of the flies that stay in the middle versus the Weighted PIs

Slope = 0.0053
Intercept = 0.240
R square value = -0.03834

contrary to the expectations, there seems to be no correlation .

T-Maze experiments : screen results as on 02-07-2018

on Monday, July 2nd, 2018 2:09 | by

Yellow 1 (Positive Control): Gr28bd-G4, TrpA1-G4

Parameters:
Light: intensity (500 Lux side, 1000 Lux bottom)

frequency = 20Hz

Delay = 1 ms

Duration = 9.9 ms

volts = 6.4

T-Maze experiments : screen results as on 25-06-2018

on Monday, June 25th, 2018 1:13 | by

Yellow 1 (Positive Control):  Gr28bd-G4, TrpA1-G4

Parameters:
Light: intensity (500 Lux side, 1000 Lux bottom)

frequency = 20Hz

Delay = 1 ms

Duration = 9.9 ms

volts = 6.4

Initial screen results

on Monday, June 18th, 2018 1:15 | by

Yellow 1 (Positive Control):  Gr28bd-G4, TrpA1-G4

Light: intensity (500 Lux side, 1000 Lux bottom), frequency (20Hz)

Tmaze experiments : Test for Blindness and Comparison with positive control

on Monday, June 11th, 2018 12:45 | by

Comparison between White 1 ( Control (NorpA- UAS Chrimson)) and Yellow 1 (Positive Control ( Gr28bd-G4, TrpA1-G4).

To test whether the flies are really blind, and there is no problem with the NorpA part of the construct, we compare with NorpA,UAS GTACR1 ; NorpA,UAS GTACR2 ; and another stock of NorpA-UAS-Chrimson.

 

Weighted mean is calculated by multiplying the weights (total number of flies in that experiment/total number of flies in all the experiments) with the PI for that experiment, and taking the sum thereafter for all the experiments.

Tmaze experiments initial results

on Monday, June 4th, 2018 2:16 | by

the first graph shows Bar plot of Mean and standard deviation of PIs for Genetic control (n=9) and Positive control (n=5).
The second graph shows weighted mean and weighted standard deviation of the same.

Weighted mean is calculated by multiplying the weights (total number of flies in that experiment/total number of flies in all the experiments) with the PI for that experiment, and taking the sum thereafter for all the experiments.

Optogenetics – My results vs. Lena’s results

on Monday, November 23rd, 2015 2:42 | by

 

 

 

Last week I continued the experiments for the optogenetics. These are my results:

LM2311 whole

and these are Lena’s results:

Lena

 

Compared to Lena’s results, my results are a bit too positive.

Optogenetics (Chrimson) with the Tmaze

on Friday, November 20th, 2015 5:33 | by

 

PI as a whole LM 1611 PI I LM 1611 PI II LM 1611

Last week, I started with the optogenetic experiment. Two days before the experiment,  I put male flies into little vials (lightproof) containing standard food medium and I added a dab of yeast mixed with eihter ethanol (for control group) or ATR (= all-trans-retinal) (for experiment group).

These are the experimental conditions:

~ 1600 lx; 20 Hz; 10 ms puls width; short tubes (transparent tube with red light and an opaque dark tube); 30 sec in the elevator; 1 min testing; 4 hours between the first testing and the repetition;