aPKCdelta blind half time experiment

on Monday, May 23rd, 2022 1:19 | by

Progress report on the aPKCdelta experiment with half the time (1min period)
Blind experiment with Group A&B; One with Ru486 and one without

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Test group for 1 min experiments

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Progress report for the test group in the 1 min. period experiments
UASaPKC x GSnsyb control flies

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Control Groups With and Without ATR

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NorAUASChrimsonxGr28bdTrpA1 cross has been used as a control group for joystick experiments. There are 5 different groups for controls.

1st Group: Yellow light, NO ATR

2nd Group: Red light, NO ATR

3rd Group: Yellow light, with ATR

4th Group: Red light, with ATR(2 out of 3 lights have issues)

5th Group: Red light, with ATR(all lambs are working fine for the first time)

6th Group: Red light, with ATR(higher light intensity)

7th Group: Yellow light, with ATR

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Baz knockout

on Wednesday, April 20th, 2022 8:20 | by

Baz was knocked out in all neurons in the adult fly using the gene switch system.

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Overlap FoxP – motor neuros/aPKC

on Monday, March 14th, 2022 11:40 | by

Overlap of FoxP (red) with aPKC or 3 motor neuro lines (D42, C380, OK6) (green)

aPKC

D42

C380

OK6

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Course

on Wednesday, February 16th, 2022 11:29 | by

Blocking with tetoxE

on Monday, December 20th, 2021 1:37 | by

tetoxE was crossed to different Gal4-lines. 20A02 was tested separately before, 52B10, 55G08 and 64H04 were tested in parallel.

PB expression: 52B10, 55G08

EB expression: 20A02, 64H04

Previous experiment (Posted 30.08.21):

New test

PB: 52B10, 55G08
EB: 64H04
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T-Maze experiment with red light

on Monday, December 13th, 2021 12:33 | by

T-Maze experiment with Red light:

Graph shows “Mean Performance Index” for NorpA-UAS-Chrimson/Gr28bd, NorpA-UAS-Chrimson/0273, NorpA-UAS-Chrimson/38764, NorpA-UAS-Chrimson/66555, NorpA-UAS-Chrimson/66010, NorpA-UAS-Chrimson/66009 (ATR-treated foods for 2days) and Controls (NorpA-UAS-Chrimson/Gr28bd, without ATR).

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aPKCdelta adult expression

Expressing aPKCdelta in all neurons in the adult fly.

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aPKCdelta adult expression half the experiment time

Expression of aPKCdelta in all neurons in the adult fly (nysbGS-Gal4) for half the experiment time (1 min periods).

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