FoxP aPKC schematics

on Monday, June 7th, 2021 8:16 | by

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aPKC knockout update

on Tuesday, May 25th, 2021 1:52 | by

aPKC was knock out using CRISPR Cas9 mediated editing.

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GMR55G08 (PCB) blocking

on Monday, May 17th, 2021 1:15 | by

Blocking of the line GMR55G08 (PCB) with Tetox and Kir.

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aPKC knock-out

aPKC was knock out using CRISPR Cas9 mediated editing.

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PKC gRNA

on Monday, April 12th, 2021 11:07 | by

Comparison of the kock out of aPKC and PKC53e in adutl flies.

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Problems with PKCi

ElavGal4;tubGal80 X UASPKCi were raised at 18°C. Before the experiment they spent 4h at supposedly 35°C. Since they still show a stong learning phenotype the temperatur might have not been right.

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Test of 7 days old flies after GS

on Monday, February 15th, 2021 9:35 | by

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Local FoxP knok out GMR11F02-GAL4

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Larval food manipulation evaluation

on Monday, February 8th, 2021 1:28 | by

1. Food Patch

No significant difference between groups in Standard (wilcox.test, p =0.9442), Sugar (wilcox.test, p=0.3097) and Fat (wilcox.test, p-value = 0.06267).
Significant difference between Rover and Sitter in Standard_new (wilcox.test, p=0.01057), Carbohydrates (wilcox.test, p= 0.0002445), Starch (wilcox.test, p-value = 0.03431) and Yeast (wilcox.test, p = 0.001455)

2. Distance Tracking

Signifcant difference between Groups in Standard (t-test, p=0.0317), Sugar (t-test, p=0.0103), Fat (t-test, p=0.01823), Starch (t-test, p = 0.03895) and Yeast (t-test, p=0.001894).
No significant difference between Rover and Sitter in Standard_new (t-test, p =0.08108), Carbohydrates (t-test, p=0.09875),
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Larval food manipulation first evaluation

on Monday, February 1st, 2021 12:40 | by

Food Patch Experiment

The development of larvae that grew up on food without yeast was delayed by about 2 days. Larvae grown on starch feed developed faster by a day.
The standard food was probably too dry.

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