on Monday, April 15th, 2019 2:41 | by Miroslav Stojic
Final Stats (flew more than 8min/out of):
– Wild type: 14/17 (82%)
– ElavGal4 x UAS-Ryr 28919: 3/13 (23%)
– ElavGal4 x UAS-Ryr 65885: 13/100 (13%)
ElavGal4 x UASRyR 65885
on Monday, April 8th, 2019 1:14 | by Miroslav Stojic
Last week (1.-7.Apr 2019) we noted how many flies want to fly more than 8 min and here is the result (only the ones we noted):
– Wild type: 8/9 (88%)
– ElavGal4 x UAS-Ryr 28919: 3/12 (25%)
– ElavGal4 x UAS-Ryr 65885: 6/35 (17%)
Trace (Downsampled 5) Graphs of Recordings
ElavGal4 x UAS-Ryr 28919:
ElavGal4 x UAS-Ryr 65885:
on Monday, April 1st, 2019 2:54 | by Miroslav Stojic
Elav-Gal4 x UAS-SERCA 44581 flies => Lethal before adulthood! (in all 5 vials so far)
on Monday, March 25th, 2019 12:49 | by Miroslav Stojic
And the journey begins…
ElavGal4 x UASRyr65 “the wrong vials”
– > Graphs of the Trace.png (RightTrace – LeftTrace):
on Monday, July 30th, 2018 1:52 | by Anokhi Kashiparekh
on Monday, July 23rd, 2018 1:55 | by Anokhi Kashiparekh
on Monday, July 16th, 2018 1:46 | by Anokhi Kashiparekh
The text file looks something like this: Not very sure how to interpret it because there is no column header.
> Did not find any RU486 fly lines in the Brembs fly stock.
What is RU486 and why are we using it? It is a conditional transactivation method that gets activated when introduced with Mifepristone/RU486 and works on the UAS promoter (Roman et al, 2001).
The genes we are knocking down:
1) SERCA gene
2) Ryr gene
on Friday, May 18th, 2018 3:34 | by Christian Rohrsen
This is a picture of the supplemental figure from Maye et al. 2007
on Tuesday, May 15th, 2018 12:26 | by Christian Rohrsen
and at 40x
In this link we have a video of a 3D stainning pattern zoomed_CC
on Monday, May 14th, 2018 11:22 | by Christian Rohrsen
Axel stainning from both drivers together. I would say it really contains both driver lines.
This are my stainnings at the fluorescence microscope (no confocal). This is to show that in all of the 10-12 brains I have looked at, they all had the c232 pattern present
In addition, they had many more neurons outside from the central complex which I believe belong to the c105-G4 line. This is my only proof to show that c105 is also present, since the R1 neurons seem to be hidden when R2 neurons are stained.