Strokelitude – Final Work

on Monday, April 15th, 2019 2:41 | by

Final Stats (flew more than 8min/out of):
– Wild type: 14/17 (82%)
– ElavGal4 x UAS-Ryr 28919: 3/13 (23%)
– ElavGal4 x UAS-Ryr 65885: 13/100 (13%)

Wild Types:

ElavGal4 x UASRyR 65885

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Strokelitude – New Findings and Recordings (1.-7. Apr.)

on Monday, April 8th, 2019 1:14 | by

Last week (1.-7.Apr 2019) we noted how many flies want to fly more than 8 min and here is the result (only the ones we noted):
– Wild type: 8/9 (88%)
– ElavGal4 x UAS-Ryr 28919: 3/12 (25%)
– ElavGal4 x UAS-Ryr 65885: 6/35 (17%)

Trace (Downsampled 5) Graphs of Recordings

Wild type:

ElavGal4 x UAS-Ryr 28919:

ElavGal4 x UAS-Ryr 65885:

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Strokelitude 5 New Rec

on Monday, April 1st, 2019 2:54 | by

New Findings:

Elav-Gal4 x UAS-SERCA 44581 flies => Lethal before adulthood! (in all 5 vials so far)

Elav-Gal4 x Ryr 65885
Elav-Gal4 x Ryr 28919
Elav-Gal4 x Ryr 28919
Elav-Gal4 x Ryr 28919
Elav-Gal4 x Ryr 28919
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Strok(e)litude – Practice

on Monday, March 25th, 2019 12:49 | by

And the journey begins…

ElavGal4 x UASRyr65 “the wrong vials”
– > Graphs of the Trace.png (RightTrace – LeftTrace):

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Stroklitude Testing Pt. 2

on Monday, July 30th, 2018 1:52 | by

Data 1:

Monica:

Anokhi:

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Stroklitude Data

on Monday, July 23rd, 2018 1:55 | by

This week we worked on perfecting the fly hooking. Some of the data we got is as follows (Data from just one fly).

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Experiment Update

on Monday, July 16th, 2018 1:46 | by

> Worked on the ping pong ball machine in the last week.
IMG_2907 (1)

The text file looks something like this: Not very sure how to interpret it because there is no column header.

> Did not find any RU486 fly lines in the Brembs fly stock.
What is RU486 and why are we using it? It is a conditional transactivation method that gets activated when introduced with Mifepristone/RU486 and works on the UAS promoter (Roman et al, 2001).

The genes we are knocking down:
1) SERCA gene
2) Ryr gene

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Sathish scripts in my hands are reproducing results

on Friday, May 18th, 2018 3:34 | by

 

 

This is a picture of the supplemental figure from Maye et al. 2007

 

Below the results from the sathish scripts running on the data from Maye et al. 2007. It matches, so Sathish scripts in my hands work fine

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Confocal images and boxplots from my results in strokelitude

on Tuesday, May 15th, 2018 12:26 | by

Confocal image MAX stack of one of the brains at 20x

 

 

and at 40x

 

In this link we have a video of a 3D stainning pattern          zoomed_CC

 

In addition I add here teh boxplots from the final results of the Ping Pong ball setup with these experiments

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Stainning c105;;c232

on Monday, May 14th, 2018 11:22 | by

 

The first figure shows each of the central complex ring neurons types (Martín Pena et al., 2014). The c105-G4 targets the R1 neurons and the c232-G4 targets the R2 and the R4d neurons

This is the c105-G4 stainning from Martín Pena et al., 2014

232-G4 stainning from Kahsai et al., 2012

 

Axel stainning from c232-G4 alone

Axel stainning from c105-G4 alone

Axel stainning from both drivers together. I would say it really contains both driver lines.

This are both driver lines together as well from Axel. To me it seems that only c105 is present

This are my stainnings at the fluorescence microscope (no confocal). This is to show that in all of the 10-12 brains I have looked at, they all had the c232 pattern present

In addition, they had many more neurons outside from the central complex which I believe belong to the c105-G4 line. This is my only proof to show that c105 is also present, since the R1 neurons seem to be hidden when R2 neurons are stained.

I was also looking to the youtube video you have online, Björn. To me it seems I can only see the R1 ring neuron from the c105

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