Bachelor Blog / #1 outline

on Monday, May 22nd, 2023 8:45 | by

Hello everyone!

Here is a brief overview of the topics I will be dealing with over the next few months:

This is the experiment by MD Ziv Williams I am trying to imitate ->

If you are further interested in transgenerational inheritance of behavioral traits and non synaptic memory storage check out these interesting links ->

This week, for now, I will continue practicing to glue the flies and make a realistic estimate of the number of flies I need to get a significant result. Hopefully I can start trying out my experimental setup by the start of next week :)

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Strokelitude – Final Work

on Monday, April 15th, 2019 2:41 | by

Final Stats (flew more than 8min/out of):
– Wild type: 14/17 (82%)
– ElavGal4 x UAS-Ryr 28919: 3/13 (23%)
– ElavGal4 x UAS-Ryr 65885: 13/100 (13%)

Wild Types:

ElavGal4 x UASRyR 65885

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Strokelitude – New Findings and Recordings (1.-7. Apr.)

on Monday, April 8th, 2019 1:14 | by

Last week (1.-7.Apr 2019) we noted how many flies want to fly more than 8 min and here is the result (only the ones we noted):
– Wild type: 8/9 (88%)
– ElavGal4 x UAS-Ryr 28919: 3/12 (25%)
– ElavGal4 x UAS-Ryr 65885: 6/35 (17%)

Trace (Downsampled 5) Graphs of Recordings

Wild type:

ElavGal4 x UAS-Ryr 28919:

ElavGal4 x UAS-Ryr 65885:

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Strokelitude 5 New Rec

on Monday, April 1st, 2019 2:54 | by

New Findings:

Elav-Gal4 x UAS-SERCA 44581 flies => Lethal before adulthood! (in all 5 vials so far)

Elav-Gal4 x Ryr 65885
Elav-Gal4 x Ryr 28919
Elav-Gal4 x Ryr 28919
Elav-Gal4 x Ryr 28919
Elav-Gal4 x Ryr 28919
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Strok(e)litude – Practice

on Monday, March 25th, 2019 12:49 | by

And the journey begins…

ElavGal4 x UASRyr65 “the wrong vials”
– > Graphs of the Trace.png (RightTrace – LeftTrace):

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Stroklitude Testing Pt. 2

on Monday, July 30th, 2018 1:52 | by

Data 1:

Monica:

Anokhi:

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Stroklitude Data

on Monday, July 23rd, 2018 1:55 | by

This week we worked on perfecting the fly hooking. Some of the data we got is as follows (Data from just one fly).

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Experiment Update

on Monday, July 16th, 2018 1:46 | by

> Worked on the ping pong ball machine in the last week.
IMG_2907 (1)

The text file looks something like this: Not very sure how to interpret it because there is no column header.

> Did not find any RU486 fly lines in the Brembs fly stock.
What is RU486 and why are we using it? It is a conditional transactivation method that gets activated when introduced with Mifepristone/RU486 and works on the UAS promoter (Roman et al, 2001).

The genes we are knocking down:
1) SERCA gene
2) Ryr gene

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Sathish scripts in my hands are reproducing results

on Friday, May 18th, 2018 3:34 | by

 

 

This is a picture of the supplemental figure from Maye et al. 2007

 

Below the results from the sathish scripts running on the data from Maye et al. 2007. It matches, so Sathish scripts in my hands work fine

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Confocal images and boxplots from my results in strokelitude

on Tuesday, May 15th, 2018 12:26 | by

Confocal image MAX stack of one of the brains at 20x

 

 

and at 40x

 

In this link we have a video of a 3D stainning pattern          zoomed_CC

 

In addition I add here teh boxplots from the final results of the Ping Pong ball setup with these experiments

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