I wanted to expand and look into some general behavior of the mbon-2 flies. Mostly as a complement to the already existing data.

Updates in DTS code

Refreshing dissection skills

LMC L1 and L2 Paper

(Coombe, P.E., 1986)

ERG

1. mass electrical response of the eye
2. waveform consisting of summed extracellular potentials produced by retinula cells and postsynaptic neurons
3. recorded by electrodes
4. waveform consists of on transient, negative sustained potential and off transient

L1 and L2

1. Two main reasons to select study upon these two:
   1. Only lamina neurons which are known electrophysiology
   2. Intracellular waveforms roughly correspond to ERG transients

Vam mutants

1. Vam = Vacuolar medulla
2. Semi-dominant mutation (Incomplete dominance)
3. Characterized by formation of large vacuoles and absence of ERG transients

(Electron Micrographs from the lamina) (ERG)

ERG waveform and LMC degeneration

1. No wt showed degeneration of LMC
2. Negative nonlinear relationship between % of LMC degeneration and the size of on/off transients in Vam flies

 

Results

1. Previous work has shown age-specific degeneration of LMC in the lamina.
2. Signs of degeneration start to appear in the form of large vacuoles in medulla and lamina.
3. LMC may be responsible for ERG transients

There are two strains of drosophila prepared: PKG-rover(R), PKG-sitter(S). Each strain is supposed to test PI in 3 phases: no training, training, long term+no training. The following data show PKG-sitter flying trace and PI. Since there weren’t many flies in the first week, and they pause flying very often in the experiment, so there is only one single fly was tested successfully. Since the torque meter drifted on the next day, the long term result is quite debatable.

 

 

Just came across this all-time classic paper from 2007. Every student of biology should be familiar with it!