Optomotor response | .:lab.brembs.net:.

Statistical evaluation of OR

on Monday, May 25th, 2020 12:23 | by Anders Eriksson

Recently I measured the optomotor response in T4/T5 flies. As expected, they did not respond to the optomotor stimulus as seen in the left chart below. However, statistical evaluation struggles to quantify this difference. It might be that this is because of the low sample size, or that we are using the wrong statistical analysis?

Category: crosses, genetics, Lab, Optomotor response, R code | No Comments

Motion blindness in T4/T5 using Optomotor Response

on Tuesday, March 31st, 2020 1:48 | by Anders Eriksson

T4/T5 driver line was crossed TNT line to test for motion blindness using the optomoter response assay. While the controls respond perfectly fine to the rotating striped arena the T4/T5 flies are seemingly unaware of it. Sample size for T4/T5 flies is still a bit low. However, as this is only a trial experiment for the drosophila course I consider it successful.

Category: genetics, Lab, Optomotor response | No Comments

Optomotor response works consistently with old platform

on Friday, March 20th, 2020 3:23 | by Anders Eriksson

The old platform works a lot more consistently compared to the new one. Unless major improvements are seen I do not see any reason to update it.

Category: Optomotor response | No Comments

Updates on optomotor response software

on Friday, March 20th, 2020 3:19 | by Anders Eriksson

Added a timer
Previous version generated faulty xml files. This bug has been squashed (see image below)
Display window of the software now also prints which direction the striped arena turns

The highlighted red areas are the ones that caused issues. Contingency has also been added to the xml file from the optomotor response (not shown). This did not cause any errors when running the DTS analysis Rscript but I thought it looked nicer having it there.

Category: Optomotor response, R code, Uncategorized | No Comments

New optomotor platform not yet optimized

on Monday, March 16th, 2020 1:42 | by Anders Eriksson

A new platform have been designed for the optomotor response. It is placed on a pivot with the axis attached on the back. In contrast to the previous design witch only monitored movement in the left/right direction, the new design in theory allows the fly to rotate the platform.