on Tuesday, March 31st, 2020 7:40 | by Björn Brembs
I’ve started rescreening Christian’s most consistent dopaminergic lines with the positive controls at the joystick machine, to see if everything works. These flies express the optogenetic channel Chrimson in Gr28bd and TrpA1 heat sensitive neurons, so they should use the joystick to switch the light off.
I tested three rounds of three flies each and I got the impression that it worked while I was looking at the flies. The data didn’t look that great, however:
Setting up the experiments, I noticed that the middle setup didn’t really focus the light on the head of the fly very well. This shows in the raw performance index data:
The two middle flies (row #2 and #8) have positive preferences, but never avoid the light, while nearly all other flies do avoid the light to various extents, even if they start out with a positive preference. So I deleted these two flies to see how the data would look then:
I decided that this should be good enough. I improved the middle setup to focus the light better on the fly’s head. Hopefully it will improve the PIs of the flies placed there now.
on Wednesday, February 19th, 2020 1:45 | by Anders Eriksson
The figure below contains the separate data from both Emer, and Ferruh/Kader. Both data sets have been merged too.
|Fly line||sample size|
on Monday, October 8th, 2018 2:44 | by Christian Rohrsen
on Friday, August 31st, 2018 4:23 | by Christian Rohrsen
For testing correlations among setups, and similarly for doing a PCA one needs a full matrix, and not a sparse matrix as I have. What are the options? a few…
There is LSEOF (Empirical Orthogonal Function Analysis), RSEOF which is like LSEOF but recursive and normally achieves better results. There is another algorithm that is called DINEOF which consists of an additional step, interpolation, before doing LSEOF. The latter has shown to yield the best results. That is why I opted for this for filling my matrix for further analysis.
The results are shown in the figure below
Here the correlation of the mean results for the lines tested in the experiments. From the correlation numbers I would not say that there is any clear correlation of any experiment to the other ( Adj R² = 0.1392 for Joystick and Y-mazes; Adj R² = 0.06809 for Joystick and yellow T-maze)
Even if there is no clear correlation among setups that the PCA could use, I decided to do a PCA to see what comes out of it. In the table below one can see the PCA loadings from the 4 different setup. We see that the PC1 uses a positive correlation of Joystick,Ymazes and yellow T-maze. PC2 a negative correlation of red T-maze and Joystick and positive Y-mazes and yellow T-mazes. After all there is not much to say about this, I think.
This is how the table of “discrete phenotypes” looks like. The first column is positive scores and the second is negative scores. That means that we are looking for lines that have 4´s. This means that it has 4 negative/positive scores (Joystick, Ymazes, Tmaze red and yellow). In this case TH-G1 and TH-D1 have 4 positive scores (HL9 and TH-C1 have 3). TH-D’ have 4 negative scores (MB025B has 3). Unfortunately not all lines were tested in all setups so we might miss some interesting lines.
This is an histogram of the amount of zeros, ones,… that are in the above table.
The histogram below is the same as above but by generating surrogate data. I just sampled data from each experiment and checked what this imaginary lines might do. The histogram is quite similar to the one above. I think that having the same histogram shape only shows that there is not correlation of effects among setups, which was already shown above.
This is another way of looking for the interesting lines. I decided to withen the data so that all have mean zero and equal deviation so that all experiments have same weight. Then I did a mean for all experiments for each line. The extreme values show that they had a very strong overall phenotype. Here one can confirm what was seen with the “discrete phenotypes”. TH-D’ shows a negative score. TH-D1 a positive one, as well as MB060B and HL9.
Here we plot in different colours the effect from each of the setups tested. Here are also lines like TH-D’ and TH-D1 outstanding. I think it is important to see that the longer the bars the most interesting, but also the more mixed contribution of each experiment the more stronger the statistics might be.
on Tuesday, August 21st, 2018 2:50 | by Christian Rohrsen
from Galili et al. 2014:
from the table in B, the expression of TH-D’-G4 is different from TH-F3-G4 in PPL1 regions projecting to alpha and alpha’ as well to dorsal Fan Shaped Body (dFB) and DP. I would not focus on the alpha projections into the Mushroom bodies (MB), because the other G4s targeting the alpha lobes did not yield any effect.
In PPM3 only is different the projection to the ellipsoid body (EB). In addition from graph A, PPM1 and PAL regions are stained by TH-D’ but might not be targeted by the other drivers shown.
from Liu et al. 2012:
We see that other drivers that were also tested in our screen (like TH-D4 and TH-G1) also stain the PPL1->dFB, PPL1->DP and PPM3->EB. Since these two drivers did not have a phenotyp,e we might not attribute the effect of TH-D’ because of these projections.
From Pathak et al. 2015:
We have a different pattern where they do not describe expression in regions like PPM2, PPM1 or PAL. They point out the expression in PPM3 and PPL1 but we already discarded these regions as the ones involved in reinforcement in the graphs above in this post. They also observed expression in PPL2, which might be a region also stained by TH-G4, TH-D1 and TH-C’, but we do not know how they overlap. A few TH+ neurons in the ventral ganglia are also targeted by TH-D’.
From White et al. 2011?:
We see more general dopaminergic anatomical properties, like the number of neurons in each dopaminergic cluster. In the second graph one can see where the PPL1, PPM3, PPM1/2 and PAL project to.
from Xie et al. 2018:
I would say that the only two interesting columns are 1&2 and 2&3 which finds common regions for TH-C vs TH-D and TH-D vs TH-F, respectively. The only conclusion I would take from the first is that a few PPM2 regions are discarded as interesting, and from the second that the whole PPL1 does not seem to have differential expression in TH-D’.
on | by Christian Rohrsen
These are the experiments from Amanda, the second plot is the amount of experiments per line. Since the positive control does not show the aversive phenotype I am afraid I have to throw her data away :(
I can´t see if effects reproduce in different hands because I do not have the data from Amanda, so I will just pool all the data together.
on Tuesday, August 14th, 2018 2:40 | by Christian Rohrsen
Estimate Std. Error t value Pr(>|t|)
(Intercept) 0.05663 0.12282 0.461 0.661
new_Christian$mean 1.07180 0.56922 1.883 0.109
Residual standard error: 0.1943 on 6 degrees of freedom
Multiple R-squared: 0.3714, Adjusted R-squared: 0.2667
F-statistic: 3.545 on 1 and 6 DF, p-value: 0.1087
Estimate Std. Error t value Pr(>|t|)
(Intercept) -0.05975 0.03595 -1.662 0.1101
new_Gaia$mean 0.33806 0.19627 1.722 0.0984 .
Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1
Residual standard error: 0.1787 on 23 degrees of freedom
Multiple R-squared: 0.1142, Adjusted R-squared: 0.07574
F-statistic: 2.967 on 1 and 23 DF, p-value: 0.09842
on | by Christian Rohrsen
here the final screen
Here a total of 37 Tmaze experiments containing NorpA with UAS-GtACRs, UAS-Chrimson or UAS-ChR2XXL but without G4. Experiments are from Naman, Gaia and me, here the pooled effect:
Here the barplot of the result of 12 samples (with repetitions) for 20 times. Considering 37 experiments are closer to the real true distribution of NorpA flies, we sample from them to observe the probability of obtaining false positives, as well as the distributions.
Here the boxplotFrom this results I would deduce that most of these neurons have no effect on their reinforcement. This idea came because I saw that the phenotype scores of th-G4+th-G80>Chrimson was always close to zero in all of the behavioral setups. I thought that this could show that the dopaminergic neurons have an effect that is context dependent and that is why PIs might be more extreme than with the negative control (th-G4+th-G80>Chrimson).
This is a quick edit to see how it would look with 32 lines (the same number as for the real screen)
on Monday, July 30th, 2018 2:08 | by Amanda Torres
on Friday, July 27th, 2018 3:54 | by Gaia Bianchini
Positive Control: Gr28bd-G4, TrpA1-G4
Parameters: Light: intensity (500 Lux side, 1000 Lux bottom); frequency = 20Hz; Delay = 1 ms; Duration = 9.9 ms; volts = 6.4
Red lines: completed
mb025b: not selected against tubby